槲皮素、EGCG对胆固醇酯酶活性和胆固醇胶束抑制作用研究  

Inhibition of pancreatic cholesterol esterase activities and cholesterol micelle of EGCG and quercetin

作　　者：苏建辉[1] 马朝阳[1] 杨鹿[1] 王洪新[1,2] 高传忠 聂荣京 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学国家功能食品工程技术研究中心,江苏无锡214122 [3]铜陵瑞璞牡丹产业发展有限公司,安徽铜陵244000

出　　处：《食品工业科技》2015年第11期346-349,共4页Science and Technology of Food Industry

基　　金：国家125项目(2012BAD33B05);功能食品设计及制造关键技术与产品;安徽铜陵瑞璞牡丹产业发展有限公司合作项目

摘　　要：为降低胆固醇酯类水解及小肠对胆固醇的吸收,本实验体外模拟人体小肠内环境,研究EGCG和槲皮素对胆固醇酯酶活性及胆固醇胶束溶解度的抑制效果。同时比较了9种功能因子对胆固醇酯酶活性及胆固醇胶束溶解度的抑制效果。结果表明,槲皮素和EGCG均降低了胆固醇胶束溶解度,IC50值分别是2.2、0.08mg/m L,其抑制效果呈浓度依赖性;槲皮素和EGCG对胆固醇酯酶活性的IC50值分别是0.27、1.43mg/m L。槲皮素对胆固醇胶束溶解度的抑制效果小于EGCG,但是对胆固醇酯酶活性的抑制效果大于EGCG。结果初步证明了,槲皮素和EGCG延迟了餐后高胆固醇形成,对高血脂症有一定的预防和治疗作用。The aim of the present study is to investigate the effect of EGCG and quercetin on the inhibition of pancreatic cholesterol esterase activities and cholesterol micelle formation which may result in a reduced cholesterol absorption and hydrolysis of dietary cholesterol esters. The inhibition of nine functional factors on pancreatic cholesterol esterase activities and cholesterol micelle formation were compared.The results showed that both of EGCG and quercetin had obvious effect on inhibiting cholesterol micelle formation and the 50% inhibiting concentration( IC50. mg/mL)of each was 0.08,2.2mg/mL, which was lower than chitosan.The IC50 of EGCG and quercetin on pancreatic cholesterol esterase was 1.2,0.28mg/mL. The inhibition of cholesterol micellization by quercetin was weaker than EGCG, but quercetin showed stronger pancreatic cholesterol esterase inhibitory activity.It was suggested that EGCG and quercetin may delay postprandial hypercholesterolaemia,and therefore may be applied for the prevention and treatment of hyperlipidaemia.

关 键 词：槲皮素 EGCG 胆固醇胶束 胆固醇酯酶 降脂 

分 类 号：TS201.4[轻工技术与工程—食品科学]