高效液相色谱法测定荧光假单胞菌发酵液中谷胱甘肽含量的方法研究  

Determination of glutathione in Pseudomona fluorescens by HLPC

作　　者：黄荷[1] 易弋[1] 邵辰[1] 伍时华[1] 黎娅[1] 

机构地区：[1]广西科技大学生物与化学工程学院广西糖资源绿色加工重点实验室广西高校糖资源加工重点实验室,柳州545006

出　　处：《食品科技》2015年第5期322-325,共4页Food Science and Technology

基　　金：柳州市科学研究与技术开发计划课题(2013E020602);广西自然科学基金项目(2013GXNSFBA019081)

摘　　要：建立了荧光假单胞菌发酵液中谷胱甘肽的高效液相色谱测定方法。采用Symmetry C18柱分离,流动相为磷酸二氢钠（p H=3）和0.1%的辛烷磺酸钠混合溶液：甲醇=92.5：7.5（v/v）,流速0.8 m L/min,柱温30℃,紫外检测器,检测波长210 nm。结果显示,谷胱甘肽在10~160 mg/L浓度范围内与峰面积有良好的线性关系（线性系数为0.9999）,精密度、稳定性、重复性以及加标回收率实验的RSD值均符合检测要求。方法能快捷准确检测出荧光假单胞菌发酵液中还原型谷胱甘肽的含量。In this paper a method has been established for measuring of glutathione in Pseudomona fluorescens by high performance liquid chromatography. The determination was analysis by HLPCUV with C18 column at a temperature of 30 ℃, the detection wavelength was at 210 nm. And the mobile phase were composed of a mixture of sodium dihydrogen phosphate（p H=3） with 0.1% octane sulfonic acid sodium in water and methanol（92.5：7.5, v/v） at a flow rate of 0.8 m L/min. The results indicate that the calibration curve of glutathione was in good linearity over the rang of 10~160 mg/L（R2=0.9999）. The precision of RSD conformed to the requirements of precision test, stability test, repetitive test and recovery test. In conclusion, this method is quick, accurate and very suitable to measure glutathione from fermented liquid of Pseudomona fluorescens.

关 键 词：高效液相色谱 荧光假单胞菌 紫外检测器 谷胱甘肽 

分 类 号：TS261.1[轻工技术与工程—发酵工程]