分阶段控制温度提高胞外多糖产量  

Two-Stage Temperature Controlling Strategy For Improving the Yield of Expolysaccharide

作　　者：吴明霞[1] 赵文净[1] 

机构地区：[1]宁德师范学院生物系,福建宁德352100

出　　处：《食品与生物技术学报》2015年第5期542-546,共5页Journal of Food Science and Biotechnology

基　　金：福建省教育厅A类项目(JA12341)

摘　　要：利用冬虫夏草头孢菌发酵生产胞外多糖,研究不同温度下对菌体生长和胞外多糖产量的影响。结果表明:26℃是菌体生长的最适温度,28℃是产物积累的最适温度。在此基础上,提出分阶段控制温度工艺。通过响应面法分析,得到优化方案为第一阶段温度26℃,变温时间17 h,第二阶段温度28.5℃,胞外多糖产量10.92 g/L,比28℃恒温培养提高了25.5%。The fermentation production of expolysaccharide from Cordyceps militaris was studied at different temperature. The results showed that the optimal growth temperature was at 26 ℃ and the highest yield of expolysaccharide accumlation temperature was at 28 ℃. Thus,the two-stage temperature control strategy was developed based on RSM. When pre-fermentation temperature was kept at 26 ℃ for 17 hours and changed to 28.5 ℃ ,the yield increased to 10：92 g/L which was 25.5% higher than that of fermentation at single temperature at 28 ℃.

关 键 词：分阶段控制温度工艺 胞外多糖 响应面法 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TQ281