米曲糖化酶活力测定方法的比较研究  

Comparison of the Detecting Method of Glucoamylase Activity in Rice Koji

作　　者：林艳[1] 马莹莹[1] 张宿义[2] 林秋[1] 吴赫川 杨建刚[1] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000 [2]泸州老窖股份有限公司,四川泸州646000

出　　处：《酿酒科技》2015年第6期23-27,31,共6页Liquor-Making Science & Technology

基　　金：四川省(科技厅)科技创新苗子工程(2014093);酿酒生物技术四川省重点实验室开放基金(NJ2012-13)

摘　　要：米曲在酿酒中是重要的原料之一，米曲的糖化酶活力对酒的质量有着重大的影响。首先比较了目前国内常用的几种糖化酶活力测定方法的测定结果，在此基础上，与日本清酒米曲糖化酶的测定方法进行了比较。结果表明，不同测定方法的结果差异较大，其中按白酒曲中糖化酶测定方法测定的结果为36～200 U/g曲；国标中酶制剂的糖化酶活力测定方法测得结果为144.98～318.95 U/g曲；DSN法测得结果为26.96～146.67 U/g曲；清酒米曲糖化酶活力测定法测得结果为392.04～419 U/g曲。样品量对国标中酶制剂的糖化酶测定方法的测定结果影响尤其明显，酶液提取温度30℃所测值几乎均大于40℃所测值。不同浸提方法的相同测定方法测得结果表明，用清酒米曲糖化酶的浸提法时，几种测定方法所测值均较其他两种浸提法所测值大；相同提取方法不同测定方法测得结果表明，几种测定法在不同的浸提法中均有一定的适应性。总之，清酒米曲糖化酶活力测定的方法最稳定，最能体现米曲中的糖化酶活力。另外，采用清酒米曲糖化酶浸提法（经透析）提取，DNS法测定，所得结果相对稳定，与其最接近。Rice koji is an important raw material in liquor-making and its glucoamylase activity （GC） has a major impact on liquor quality. In this paper, several commonly-used GC detecting methods at home were compared and then they were compared with Japanese Sake GC detect-ing method. The results showed that, there was great difference in GC detecting results by different GC detecting method （36~200 U/g by us-ing Baijiu（liquor） GC detecting method, 144.98~318.95 U/g by national standard GC detecting method, 26.96~146.67 U/g by 3,5-dinitrosal-icylic acid （DNS） method, and 392.04~419 U/g by Sake GC detecting method）. Sample quantity had especially evident impact on the detect-ing results by national standard GC detecting method. For all methods,GC detecting results by 30℃extraction was generally higher than those by 40℃ extraction. Glucoamylase extracted by different method but detected by the same method demonstrated Sake extraction method had higher GC detecting result than the other two extraction methods. Glucoamylase extracted by the same method but detected by different meth-od demonstrated all detecting methods had good adaptability in different extraction ways. In a word, Sake detecting method could obtain the most stable detecting results reflecting true glucoamylase activity. In addition, Sake extraction method combined with DNS detecting method could also obtain relatively stable detecting results.

关 键 词：米曲 糖化酶 白酒曲 国标 清酒曲 浸提法 测定法 

分 类 号：TS261.11[轻工技术与工程—发酵工程] TQ925.7[轻工技术与工程—食品科学与工程]