食用植物油中生育酚含量测定方法优化及分析  

Optimization of Determination Method and Analysis on Tocopherol Content in Edible Vegetable Oils

作　　者：李柰[1] 樊琛[1] 李小波[1] 龙立梅 曹学丽[1] 

机构地区：[1]北京工商大学食品学院/食品添加剂与配料北京高校工程研究中心,北京100048

出　　处：《食品科学技术学报》2015年第3期59-63,69,共6页Journal of Food Science and Technology

基　　金：北京市自然科学基金重点资助项目(KZ201410011016)

摘　　要：采用高效液相色谱法测定了8种24个植物油样本中生育酚的含量,并对生育酚的提取条件进行优化。在室温下,以甲醇为溶剂,采用超声法进行提取,料液比为1：1.25,提取3次,每次20min,采用液相色谱方法进行分析。结果表明：该方法在生育酚质量浓度为0.05 ~ 1.00 g/ L时,相关系数 R2可达到0.999 2 ~0.999 4,线性关系良好;检出限分别为α-生育酚0.5 mg/ L,γ-生育酚2.0mg/ L,δ-生育酚1.2 mg/ L;三水平加标样品的平均回收率为92.1% ~99.8%,相对标准偏差（RSD,n=3）为2.5% ~8.5%。在8种植物油中,葵花籽油中α-生育酚的质量分数较高,可达4.2349×10-4,大豆油的β/γ-生育酚、δ-生育酚及总生育酚的质量分数较高,分别为7.3713×10-4 ,1.6215×10-4,9.8836×10-4。The tocopherol contents in 8 kinds of edible vegetable oils （total 24 samples） were determined by HPLC and the extraction conditions were optimized. Tocopherol was extracted three times by the ultra- sonic method and the extraction conditions were liquid material ratio 1：1.25 and extraction time 20 min. The results showed that the correlation coefficient R2 was 0. 999 2 - 0. 999 4 within the scope of 0.05 - 1.00 g/L. The detection limits of α-tocopherol, T-tocopherol, and 8-tocopherol were 0. 5, 2. 0, 1.2 mg/L, respectively, and the recovery average was 92. 1% -99.8%. Meanwhile, the relative standard deviation （RSD, n = 3） was between 2.5% -8.5%. Sunflower seed oil had the highest α-tocopherol content （4. 234 9 × 10-4） while soybean oil had the highest β/γ-tocopherol （7. 371 3 × 10-4）, δ-tocopherol （ 1. 621 5 × 10-4） , and total tocopherols （9. 883 6 × 10 -4） contents.

关 键 词：食用植物油 生育酚 高效液相色谱法 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]