西沙诺尼果汁不同极性多酚及抗氧化活性的分析  

Antioxidant activity of polyphenols with different polarities from Xisha noni juice

作　　者：陈建国[1] 梁寒峭[1] 李雪[1] 程池[1] 

机构地区：[1]中国食品发酵工业研究院,中国工业微生物菌种保藏管理中心,北京100015

出　　处：《食品与发酵工业》2015年第2期163-166,共4页Food and Fermentation Industries

基　　金：中国食品发酵工业研究院科技发展基金(博士基金)项目(KJ14-BS-02)

摘　　要：以不同极性有机溶剂对西沙诺尼果汁多酚进行分离,以Folin-Ciocalteu法测定多酚含量,通过清除DPPH·、ABTS+·能力和总抗氧化能力的测定,对不同极性多酚抗氧化活性进行评价。结果表明:液-液萃取可以实现不同极性多酚的有效分离,西沙诺尼果汁多酚以极性酚为主,水相的抗氧化能力最强,其次为乙酸乙酯相和正丁醇相,两者无显著差异,氯仿相最弱。不同萃取物多酚含量与3种方法测得的抗氧化数据具有显著正相关性,多酚是其主要的抗氧化物质。Different polyphenol extracts were obtained from Xisha noni juice using organic solvents with different polarity. Total phenolic content was determined by Folin-ciocaheu method. Antioxidant activity was evaluated by DPPH scavenging assay, ABTS scavenging assay and total antioxidant capacity assay. It was shown that liquid-liquid extraction was a successful method for separating polyphenols with different polarities, and polar polyphenols were dominant in Xisha noni juice. Antioxidant activities of water-soluble fraction is the strongest and there was no significant difference between ethyl acetate-soluble fraction and n-butanol-soluble fraction which were stronger than chloroformsoluble fraction. Antioxidant activity of different extracts was significantly positive correlated with total phenolic content. The polyphenols were the main antioxidant substances.

关 键 词：西沙诺尼 多酚 萃取物 抗氧化活性 

分 类 号：TS255.44[轻工技术与工程—农产品加工及贮藏工程]