清香型大曲细菌群落结构的比较分析  

Comparative analysis of bacterial community structure of Chinese Fen-Daqus

作　　者：叶光斌[1,2] 王彩虹[1] 王毅 甄攀 王勇[1] 罗惠波[1,2] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000 [2]酿酒生物技术及应用四川省重点实验室,四川自贡643000 [3]五粮液集团质量检测中心,四川宜宾644000 [4]山西杏花村汾酒厂股份有限公司,山西汾阳032200

出　　处：《食品与机械》2015年第3期11-15,73,共6页Food and Machinery

基　　金：酿酒生物技术及应用四川省重点实验室项目(编号:NJ2010-05;NJ2011-05);四川理工学院校级人才引进项目(编号:2010XJKRL001)

摘　　要：通过构建细菌16SrRNA基因文库、RFLP指纹图谱分析、测序和系统发育学分析,分别对汾酒清茬曲、后火曲和红心曲的细菌群落结构进行比较。结果表明,清香型3种大曲细菌均分属于芽孢杆菌纲、放线菌纲及变形菌纲,但具体种属组成存在一定差异,其中清茬曲的优势细菌菌群为乳杆菌属和葡萄球菌属(占全部克隆子的比例分别为37.78%,22.22%);后火曲的优势细菌菌群为乳杆菌属、葡萄球菌属、芽孢杆菌属、魏斯氏菌属(占全部克隆子的比例分别为35%,16.67%%,16.67%,13.33%);红心曲的优势细菌菌群为乳杆菌属、芽孢杆菌属、高温放线菌属(占全部克隆子的比例分别为26.67%,28.89%,17.78%)。The bacterial community structure of three types FenDaqus（Qingcha Daqu,Houhuo Daqu and Hongxin Daqu）were studied through construction of bacteria 16 SrRNA gene clone library,sequencing and phylogenetic analyses.The results showed that the bacteria communities of three types Fen-Daqus were both distributed in Bacilli,Actinobacteridae,Gammaproteobacteria.But there are some specific differences in detail composition of the bacterial species among the three types Fen-Daqus.Lactobacillus and Staphylococcus were the dominant bacteria in Qingcha Daqu（the ratio to total clones were 37.78%and 22.22%separately）;Lactobacillus,Staphylococcus,Bacillus and Weissella were the dominant bacteria in Houhuo Daqu（the ratio to total clones were 35%,16.67%,16.67%,13.33% separately）;Lactobacillus,Bacillus and Thermoactinomyces were the dominant bacteria in Hongxin Daqu（the ratio to total clones were 26.67%,28.89%,17.78% separately）.

关 键 词：清香型大曲 清茬曲 后火曲 红心曲 16SrRNA基因文库 细菌群落结构 

分 类 号：TS261.1[轻工技术与工程—发酵工程]