高效液相色谱法鉴别原料乳中碳水化合物掺伪  

Identify the Carbohydrate Adulteration in Raw Milk by High Performance Liquid Chromatography

作　　者：郑向华[1] 杨自洁[2] 张青燕 龚吉军[2,3,4] 肖莲荣 邓放明[4] 

机构地区：[1]厦门出入境检验检疫局科学研究院,福建厦门361026 [2]中南林业科技大学食品科学与工程学院,湖南长沙410004 [3]湖南亚华乳业有限公司,湖南长沙410013 [4]湖南农业大学生物学博士后流动站,湖南长沙410128

出　　处：《食品研究与开发》2015年第10期70-73,共4页Food Research and Development

基　　金：国家十二五科技支撑计划课题"原料奶质量安全监控关键技术研究"(2012BAD12B04);国家质检总局科技计划项目"原料乳碳水化合物掺伪鉴别技术研究"(2013IK186);湖南省高校科技成果产业化培育项目(12CY009);中南林业科技大学"十二五"专业综合改革试点项目(2013-6)

摘　　要：以Hypersil氨基柱为分离柱,乙腈-水(80∶20)为流动相,流速为1.0 m L/min,建立了用于同时鉴别原料乳中果糖、葡萄糖、蔗糖和乳糖掺伪的高效液相色谱法。结果表明,在0.5 mg/m L^20 mg/m L的范围内,线性关系良好,用于果糖、葡萄糖、蔗糖和乳糖掺伪鉴别的精密度分别为2.100%、1.500%、1.680%和2.975%,回收率分别为101.0%、100.0%、99.3%、99.3%,检出限分别0.21、0.25、0.22、0.20 mg/m L。此法准确可靠,可用于原料乳中此4种碳水化合物的掺伪检测。Taken Hypersil amino column as the separation column, CH3CN/H2O （80∶20, v/v） as the mobile phase, and the flow rate kept at 1.0 mL/min, a method of high performance liquid chromatography monitored by refractive index dectetor was established for the adulteration detection of fructose, glucose, sucrose, and lactose in raw milk. The linear relationship of these four regression equations obtained in this study was very good in a range of 0.5 mg/mL-20 mg/mL;the relative standard deviation was 2.100%, 1.500%, 1.680%, and 2.975%, respectively;the average recovery rate was 101.0%, 100.0%, 99.3%, and 99.3%, respectively;the detection limit was 0.21, 0.25, 0.22, and 0.20 mg/mL, respectively. It might be concluded that this method was an accurate and raliable one for the adulteration detection of four carbohydrates above-mentioned in raw milk.

关 键 词：原料乳 碳水化合物 掺伪检测 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] TS252.2[理学—化学]