调控爪哇正青霉生物合成8-异戊烯基柚皮素的关键酶鉴定及性质研究  

Research on the Identification and Characterization of Key Enzyme in the Synthesis of8- Prenyl Naringenin by Biological Control of Java Eupenicillium

作　　者：董亚晨[1] 张俊[1] 傅明亮[1] 范琳琳[1] 徐德钦[2] 陈启和[1] 

机构地区：[1]浙江大学食品科学与营养系,杭州310058 [2]浙江省丽水学院化学与生命科学学院,浙江丽水323000

出　　处：《中国食品学报》2015年第5期194-199,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省自然科学基金项目(No.Y12C200022);浙江省杰出青年基金项目(RI3C200003)

摘　　要：采用对硝基苯酚法建立爪哇正青霉生物转化合成8-异戊烯基柚皮素体系中O-脱甲基酶酶活的分光光度测定方法;研究生物催化合成8-异戊烯基柚皮素的过程;采用3种经典的细胞色素P450酶抑制剂对P450酶和生物转化反应之间的关系进行鉴定;研究O-脱甲基酶的酶学性质。研究结果表明,生物转化体系中O-脱甲基酶酶活力在第4天达到最大值,其最适反应温度60℃,最适p H 8.0。In this paper, a spectrophotometric method for enzymatic activity determination of O-demethylase in the 8-isopentenyl naringenin biotransformation system was established with the nitrophenol method. The biological catalysis synthesis course of 8-prenyl naringenin was studied, and then the relationship between P450 enzyme and the biotransfor- mation was indentified by using three classical cytochrome P450 enzymes inhibitors. Furthermore, the properties of O- demethylase were examined. It can be concluded that the highest activity of O-demethylase appeared on the fourth day; the optimum temperature for O-demethylase was 60 ℃, and the optimum pH was 8.0 in the biotransformation system.

关 键 词：O-脱甲基酶的酶 8-异戊烯基柚皮素 生物转化 

分 类 号：O657.3[理学—分析化学] TQ925[理学—化学]