酿酒酵母转氨酶Ⅰ基因的克隆及序列分析  

Cloning and sequence analysis of aromatic aminotransferaseⅠgene from Saccharomyces cerevisiae

作　　者：刘晓蓉[1,2,3] 郭丽琼 林俊芳[1,2] 康林芝 徐晓炜[1,2] 文源华 陈宾 黎颖茵 

机构地区：[1]华南农业大学食品生物技术研究所 [2]华南农业大学食品学院,广东广州510640 [3]广东轻工职业技术学院食品系,广东广州510300 [4]广州市澳键丰泽生物科技有限公司,广东广州510760

出　　处：《食品工业科技》2015年第14期180-183,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(31272217);农业部能源植物资源与利用重点实验室开放课题

摘　　要：利用酿酒酵母YT0801的总DNA为模板,采用PCR技术克隆出转氨酶I基因的DNA序列,利用生物信息学工具对其核酸序列和蛋白序列进行分析。测序结果表明DNA序列含有一个1503bp的开放阅读框,编码500个氨基酸,推测等电点为5.81,相对分子质量为56.2ku。同源性比对结果显示,该基因及推测的氨基酸序列与已报道的ARO8基因(Gen Bank Accession No:NM 001181067.1)的同源性均为100%。此外,对其理化性质、信号肽、疏水性、亲水性、二级结构和三级结构进行了分析。该基因的成功克隆,为其功能研究和生物合成2-苯乙醇提供了分子基础。Total DNA from Saccharomyces cerevisiae YT0801 was used as the template to clone aromatic aminotransferase I gene YT0801-aro8 by PCR amplification. Bioinformatics softwares were used to analyse the DNA and protein sequence. The result of DNA sequencing showed that the amplified YT0801-aro8 had an open reading frame of 1503bp,which coded 500 amino acid residues. The molecular mass was 56.2ku and the theoretical isoelectric point was 5.81. Blastn and Blastx alignment results demonstrated that both the nucleotide sequenoe and the deduced amino acid sequence of YT0801-aro8 exhibited 100% of similarities to AR08 （GenBank Accession number： NM 001181067.1 ）. Besides,the bioinformatics, including physical and chemical properties,the signal peptide,hydrophobicity,hydrophilicity,secondary structure and tertiary structure,were analyzed. The cloning of YT0801-aro8 could provide the molecular base for study of its functions and biosynthesizing 2-phenylethanol.

关 键 词：酿酒酵母 转氨酶I 基因克隆 序列分析 

分 类 号：TS201.1[轻工技术与工程—食品科学]