通量平衡分析的进展与应用  

Development and Software Application of Flux Balance Analysis

作　　者：颜思奇 李娟[2] 方慧生[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009 [2]南京鼓楼医院血液科,江苏南京210008

出　　处：《药物生物技术》2015年第3期269-274,共6页Pharmaceutical Biotechnology

摘　　要：代谢网络是系统生物学的一个热门话题,代谢网络包含生物体内编码酶的基因以及所有已知的代谢反应。通量平衡分析是分析代谢网络中代谢物通量的数学方法,通过计算代谢网络中代谢物的通量,可以预测生物体的生长速度或某个特定代谢物的生成速率。文章综述了通量平衡分析算法的原理、建模步骤,分枝菌酸通路的通量平衡分析实例,常用的通量平衡分析软件,并从安装要求、可用性、功能、模型交换、网络可视化5个方面比较了应用通量平衡分析算法工具的特性,以指导用户选择合适的软件工具。Genome-scale metabolic networks are a hot topic in system biology. The network reconstruction contains all of the known metabolic reactions and genes that encode each enzyme in an organism. Flux balance analysis（ FBA） is a mathematical approach for analyzing the fux of metabolites through a metabolic network. FBA calculates the flux of metabolites through this metabolic network,thereby making it possible to predict the growth rate of an organism or the rate of production of a biotechnologically important metabolite. This paper reviews theories and modeling steps of flux balance analysis algorithms,flux balance analysis of mycolic acid pathways and,commonly used flux balance analysis softwares,and then compares the flux balance analysis tools,from installation requirements,availability,functionality,model interchange,network visualization,to guide the user to select the right software tools.

关 键 词：系统生物学 代谢网络 通量平衡分析 算法 软件工具 目标函数 

分 类 号：Q811.4[生物学—生物工程]