高效液相色谱法检测乳粉中活性β-内酰胺酶含量  

Qualitative Determination of Active Beta-Lactamases in Milk Powder Samples by High Performance Liquid Chromatography

作　　者：李雪芳[1] 冯卫强[1] 戴绚丽[1] 

机构地区：[1]杭州娃哈哈集团有限公司质量监控部,浙江杭州310018

出　　处：《饮料工业》2015年第3期10-12,共3页Beverage Industry

摘　　要：建立了乳粉中活性β-内酰胺酶高效液相色谱快速检测方法。利用β-内酰胺酶能酶解青霉素钾的特性,在样品中加入β-内酰胺酶的分解底物青霉素钾,酶解后,用高效液相色谱检测青霉素钾含量,通过酶解量检测样品中活性β-内酰胺酶的含量。β-内酰胺酶的浓度在0.2-1.4mg/L范围内,青霉素钾浓度的减少量与β-内酰胺酶的浓度呈较好的线性关系,方法的相关系数0.99,回收率89.0%-95.0%,相对标准偏差3.0%-3.5%。该方法检测灵敏度高、回收率和重现性良好,适用于乳粉中β-内酰胺酶的定量检测。A quick method has been developed to determine active beta-lactamase in milk power by high performance liquid chromatography. Penicillin potassium,the substrate of active beta-lactamase,was added to the samples solution.By determining the residues of penicillin potassium via high performance liquid chromatography,thereby the contents of active beta-lactamase was calculated. The method exhibited a good linearity in the mass concentration range of 0.2 -1.4 mg/L（R2=0.99）. The recoveries of active beta-lactamase achieved 89.0%-95.0% and the RSD achieved 3.0%-3.5%.With the advantages of high sensitivity,good recoveries and repeatability,the method is suitable for the determination of active beta-lactamase in milk power.

关 键 词：乳粉 Β-内酰胺酶 高效液相色谱法 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]