瑞氏木霉纤维素酶研究进展  

Research Progress on Cellulase fromTrichoderma reesei

作　　者：陈小玲[1] 龙思宇[1] 陈英[1] 张穗生[1] 陈东[1] 

机构地区：[1]广西科学院非粮生物质酶解国家重点实验室国家非粮生物质能源工程技术研究中心广西生物质产业化工程院广西生物炼制重点实验室,广西南宁530007

出　　处：《广西科学院学报》2015年第2期113-120,共8页Journal of Guangxi Academy of Sciences

基　　金：广西自然科学基金项目(2014GXNSFAA118103)资助

摘　　要：瑞氏木霉(Trichoderma reesei)产生的纤维素酶酶系全、能分泌到细胞外,是研究得最清楚的产纤维素酶的模式菌株,但是其产生的纤维素酶还不能满足工业化转化纤维素的需要。本文从纤维素酶种类与调控、提高纤维素酶活的方法、标记基因的选择等方面概述瑞氏木霉纤维素酶,分析瑞氏木霉纤维酶研究面临的问题及对策,指出提高β?葡萄糖苷酶的产量和酶活是未来研究工作的重点之一。Trichoderma reesei are well known for efficient production of cellulases and enormous protein secretory capacity,but its cellulases can not meet the requirement of industrialization.Cellulolytic enzyme systems from T.reesei contain exoglucanases,endoglucanases andβ？glucosidase,which are regulated by carbon source and controlling genes.Various methods have been used to increase the production and activity of cellulases,including mutagenesis and genetic engineering technology.Various marker genes have been used in T.reesei,including auxotroph and drug resistance marker.Cellulosic bioconversion requires the synergistic action of the three enzymatic components,among whichβ？glucosidases is the speed limiting component.

关 键 词：纤维素酶 瑞氏木霉 酶活 改造 标记基因 

分 类 号：Q933[生物学—微生物学]