脱氧雪腐镰刀菌烯醇(DON)致BALB/c小鼠的急性、亚急性和免疫毒性研究  

Study on acute,subacute and immune toxicity induced by deoxynivalenol in BALB/c mice

作　　者：杨俊花[1] 刘峰良[1,2] 刘丹[1] 罗爱琼[1] 陈慧英[1,2] 郭文博[1,2] 谢晶[2] 赵志辉[1] 

机构地区：[1]上海市农业科学院农产品质量标准与检测技术研究所,上海201403 [2]上海海洋大学食品学院,上海201306

出　　处：《上海农业学报》2015年第3期12-18,共7页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2013)第3-8号];上海市科学技术委员会技术标准专项(12DZ0502803)

摘　　要：研究了脱氧雪腐镰刀菌烯醇(Deoxynivalenol,DON)对BALB/c小鼠的毒性作用。结果表明:DON对BALB/c小鼠的LD_(50)为3.3 mg/(kg·bw)。0.6 mg/(kg·bw)和2.4 mg/(kg·bw)DON组小鼠体重和采食量显著低于对照组和0.15 mg/(kg·bw)DON组;2.4 mg/(kg·bw)DON组肝脏、肾脏和脾脏系数显著高于对照组和0.15 mg/(kg·bw)DON组。病理组织学结果显示,2.4 mg/(kg·bw)DON组肝脏组织表面有白色坏死灶,肝细胞间质出现红细胞浸润;肾脏明显肿大,肾小管管腔狭窄,肾小球萎缩。与对照组相比,2.4 mg/(kg·bw)DON组淋巴细胞百分比显著下降;单核细胞百分比、中性粒细胞百分比和红细胞压积显著升高;脾脏CD4^+/CD8^+也显著低于对照组和0.15 mg/(kg·bw)DON组。可见,2.4 mg/(kg·bw)DON可对BALB/c小鼠造成显著的肝脏、肾脏毒性以及免疫抑制作用。The toxic effect of deoxynivalenol（DON）on BALB/c mice was studied.The results showed that the acute LD50 dosage of DON was 3.3 mg/（kg·bw）.The body weight and feed intake of 0.6,2.4 mg/（kg·bw） DON treatment groups were significantly lower than those of the control and 0.15 mg/（kg·bw）DON group.Com-pared with the control and 0.15 mg/（kg·bw）DON group,the liver,spleen and kidney coefficient of 2.4 mg/（kg·bw）DON group were significantly increased.Histopathology results showed that in 2.4 mg/（kg·bw） DON group,there were white necrotic foci on liver tissue surface with erythrocyte infiltration in the cell intersti-tial.The kidney swelled obviously,the renal tubule lumen was narrow and the glomerular atrophied.The lympho-cytes percentage （LY%）significantly decreased,while the percentage of monocytes （MO%）,granulocytes （GR%）and hematocrit（HCT）significantly increased.The ratio of CD4 ＋/CD8 ＋in spleen of 2.4 mg/（kg·bw） DON group was also significantly decreased compared to the control and 0.15 mg/（kg·bw）DON group.The liver,kidney toxicity of BALB/c mice and immune inhibitory effect were caused in 2.4 mg/（kg·bw）DON group.

关 键 词：脱氧雪腐镰刀菌烯醇 BALB/C 小鼠 急性毒性 亚急性毒性 免疫毒性 

分 类 号：S858.31[农业科学—临床兽医学]