植物甾醇酯与葛根素对营养肥胖小鼠的减肥功效  

Effects of Phytosterol Ester and Puerarin on Weight Loss in Nutritionally Obese Mice

作　　者：程远渡[1] 易有金[1] 易传祝[2] 夏菠[1] 夏延斌[1] 

机构地区：[1]湖南农业大学食品科学技术学院,湖南长沙410128 [2]湖南省疾病预防控制中心,湖南长沙410000

出　　处：《食品科学》2015年第13期223-228,共6页Food Science

基　　金："十二五"国家科技支撑计划项目(2015BAD16B00-2015BAD16B01);国家自然科学基金面上项目(31071738;31000827)

摘　　要：目的:探讨植物甾醇酯和葛根素联合使用对肥胖小鼠的减肥作用。方法:饲喂高脂饲料建立小鼠肥胖模型,将肥胖小鼠按体质量分为5组(肥胖模型组、植物甾醇酯组、葛根素组、植物甾醇酯和葛根素联合组、奥利司他组),每组10只,连续灌胃6周。观察药物干预后小鼠体质量、摄食量、肥胖指数(Lee’s指数)、脂肪系数,血清总胆固醇(total cholestetol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)水平,脂肪细胞数量和大小、肝脏病变的情况。结果:与肥胖模型组相比,植物甾醇酯组、葛根素组、植物甾醇酯和葛根素联合组小鼠体质量分别降低5.41%、6.98%、12.50%;Lee’s指数分别降低3.41%、3.26%、6.25%;脂肪系数分别降低19.88%、27.41%、30.42%。3组血清TC、TG、LDL-C水平均低于肥胖模型组,其中植物甾醇酯和葛根素联合组降幅最大,分别为21.40%、37.50%、30.43%。植物甾醇酯和葛根素联合组小鼠的脂肪细胞直径变小、数量增多、肝脏脂肪性病变得到缓解。结论:植物甾醇酯和葛根素都能抑制小鼠肥胖,其中两者联合使用时减肥效果最好。Purpose：To study the weight loss effect of phytosterol ester and puerarin in obese mice.Methods：An obese mouse model was established by feeding high fat diet,and then the obese mice were divided into five groups,including model group,phytosterol ester group,puerarin group,phytosterol ester plus puerarin group,orlistat tablet group（10 mice per group）.The treatments by gavage lasted for six weeks.Then,body weight,food intake,obese index,fat index,serum total cholestetol（TC）,serum triglyceride（TG）,low density lipoprotein cholesterol（LDL-C）,high density lipoprotein cholesterol（HDL-C）,the number and diameter of fat cells and liver lesions were measured.Results：Compared with the model group,body weight in the mice from phytosterol ester group,puerarin group and combined treatment group were respectively decreased by 5.41%,6.98% and 12.50%.Obese indexes were respectively decreased by 3.41%,3.26% and 6.25%.Fat indexes were respectively decreased by 19.88%,27.41% and 30.42%.The serum levels of TC,TG and LDL-C of the groups treated with phytosterol ester and/or puerarin decreased compared to the model group,and the combined treatment group showed the largest decline in the three serum lipid parameters by 21.40%,37.50% and 30.43%,respectively.The number of fat cells in the combined treatment group was increased,and liver fatty lesion was also alleviated.Conclusion：Phytosterol ester and puerarin have weight-reducing effects on obese mice,especially when used in combination.

关 键 词：植物甾醇酯 葛根素 肥胖小鼠 减肥 

分 类 号：R915[医药卫生—微生物与生化药学]