牛乳制品中青霉素酶Alphalisa的检测方法  

Establishment of the Alphalisa Method for the Detection of Penicillinase in Milk Products

作　　者：张姜琳 聂福平[1] 杨俊[1] 李应国[1] 肖进文[1] 蔡家利[2] 袁增壮 叶自霞 王国民[1] 王昱[1] 

机构地区：[1]重庆出入境检验检疫局技术中心,重庆400020 [2]重庆理工大学药学与生物工程学院,重庆400054 [3]西南大学动物科技学院,重庆400716

出　　处：《食品科学》2015年第14期181-186,共6页Food Science

基　　金：重庆市科技攻关项目(2014yykf A80017;2010GGC239);国家质检总局质检项目(2012IK029)

摘　　要：建立牛乳制品中青霉素酶Alphalisa的检测方法。将青霉素酶进行生物素化,与样品中的青霉素酶竞争结合鼠源性青霉素酶单克隆抗体,利用Alphalisa反向竞争的反应模式,对牛乳中的青霉素酶实施检测。建立的Alphalisa检测方法能特异性识别样品中存在的青霉素酶,方法的线性范围在2.5-500 IU/mL;检出限和定量限分别为1.6 IU/mL和5 IU/mL;批间批内变异度均小于10%。结果表明,该方法能够替代传统酶联免疫吸附实验方法,成为青霉素酶的实验室筛查或检测方法。Penicillinase is one of the rick drugs present in milk products. In this article, the standard penicillinase was biotinylated, and competitively bound to mouse anti-penicillinase monoclonal antibodies by reverse competitive Alphalisa. The established Alphalisa detection method was shown to specifically identify penicillinase present in the samples. The variations between batches were less than 10%, with the limit of detection and the limit of quantitation of 1.6 IU/mL and 5 IU/mL, respectively, and the linearity range of 2.5-500 IU/mL. The results show that this method can replace the traditional ELISA method as a laboratory screening or detection method for penicillinase.

关 键 词：青霉素酶 Alphalisa检测 牛乳制品 

分 类 号：R155.5[医药卫生—营养与食品卫生学]