金属离子对巨大口蘑褐变相关酶活力的影响  

Effect of the brown enzyme activity of Tricholoma giganteum under different metal ions treatment

作　　者：魏要武[1] 莫美华[1] 陈东梅[1] 杨水莲[1] 昌毓嵩 郑锦荣[1] 

机构地区：[1]华南农业大学食品学院,广州510600

出　　处：《食品科技》2015年第7期55-60,共6页Food Science and Technology

基　　金：国家自然科学基金项目(31272218);国家星火计划项目(2012GA780042;2013GA780040);广东省农业科技推广专项(201201138);广州市花都区产学研结合专项(HD13CXY-010);广东省科技计划项目

摘　　要：巨大口蘑是一种珍稀食用菌,具有抗褐变特性,了解其褐变相关酶的特性,对探明其抗褐变机理具有重要意义。采用不同金属离子（Zn^2＋、Mg^2＋、Cd^2＋、Cu^2＋、Fe^2＋、Fe^3＋、Mn^2＋）的梯度溶液浸泡法处理蘑菇切片,并测定了不同金属离子对其褐变相关酶活力影响。结果表明：Cu^2＋、Zn^2＋、Mg^2＋对其多酚氧化酶（PPO）具有显著低促高抑作用,Fe^3＋为激活作用,Fe^2＋和Cd^2＋起抑制效果。0.4-2 mmol/L的Zn^2＋、Mg^2＋和Cd^2＋对其酪氨酸酶（TYE）有显著激活作用,Cu^2＋具有低促高抑作用;Fe^3＋、Fe^2＋和Mn^2＋对TYE活力影响不显著。Cd^2＋、Cu^2＋、Fe^3＋、Mn^2＋和Zn^2＋对其苯丙氨酸解氨酶（PAL）有显著激活效果,Fe^2＋对PAL有抑制作用;Mg^2＋对PAL无显著影响。In order to explore the mechanism of Trichoioma giganteum anti-browning,the gradient solution immersion method with different metal ions（Zn^2＋,Mg^2＋,Cd^2＋,Cu^2＋,Fe^2＋,Fe^3＋,Mn^2＋） was adopted to process mushroom slices,and the influence of different metal ions on the browning related enzyme activity was determinated in this paper.The results showed that Cu^2＋,Zn^2＋,Mg^2＋ had significant stimulation on the poiyphenoi oxidase（PPO） activity at low concentration and had inhibition at high concentration.Fe^3＋ could active PPO activity.Fe^2＋ and Cd^2＋ had inhibitory effect.0.4-2 mmol/L Zn^2＋,Mg^2＋ and Cd^2＋ had significant activation on the tyrosinase（TYE） activity.Cu^2＋ had stimulation at low concentration and had inhibition at high concentration.The influence of Fe^3＋,Fe^2＋ and Mn^2＋ on the TYE activity was not significant.Cd^2＋,Cu^2＋,Fe^3＋,Mn^2＋ and Zn^2＋ had significant activation on the phenylalanine ammonialyase（PAL） activity.Fe^2＋ had inhibition on PAL;Mg^2＋ had no significant influence on PAL activity.

关 键 词：巨大口蘑 金属离子 多酚氧化酶 酪氨酸酶 苯丙氨酸解氨酶 

分 类 号：TS201.25[轻工技术与工程—食品科学]