芡种皮多酚提取物体外抑制α-葡萄糖苷酶和α-淀粉酶活性研究  

In vitro inhibitory effects of polyphenol extracts from Euryale ferox seed coat on α-glucosidase and α-amylase activities

作　　者：伍城颖[1,2] 吴启南[3,4] 王红[4] 樊修和 

机构地区：[1]南京中医药大学附属中西医结合医院中药质量研究室,江苏南京210028 [2]中国中医科学院江苏分院和江苏省中医药研究院中药代谢组研究室,江苏南京210028 [3]江苏省中药资源产业化过程协同创新中心,江苏南京210023 [4]南京中医药大学药学院,江苏南京210023

出　　处：《食品工业科技》2015年第16期91-94,99,共5页Science and Technology of Food Industry

基　　金："十二五"国家科技支撑计划项目(2011BAI04B06);江苏高校优势学科建设工程资助项目;江苏省高等学校"中药资源化学研究"优秀科技创新团队(2011)项目;江苏省2013年度普通高校研究生科研创新计划项目(CXLX13_600)

摘　　要：通过体外实验研究了芡种皮多酚提取物对α-葡萄糖苷酶和α-淀粉酶活性的影响,并考察了其对α-葡萄糖苷酶和α-淀粉酶的抑制动力学,研究结果显示:芡种皮多酚提取物在体外具有较强的抑制α-葡萄糖苷酶和α-淀粉酶的作用(IC50分别相当于生药浓度0.08 mg/m L和0.30 mg/m L),其抑制作用与浓度之间存在量效关系,二者的抑制类型均为可逆性的竞争性抑制剂。因此,芡种皮具有开发成辅助降糖的保健食品或药品的潜力,有很大的利用价值。The inhibitory effects of total polyphenols extracts from Euryale ferox seed coat against α-glucosidase and s-amylase were studied through the in vitro testing of inhibition rate and inhibition kinetics. The results showed that the total polyphenols exhibited strong inhibitory effects against α-glucosidase and α-amylase in vitro with dose-effect relationship and the IC50 were 0.08 mg/mL and 0.30 mg/mL,respectively. The inhibition type was reversible and competitive inhibitor. In conclusion, Euryale ferox seed coat would be a potential source for a hypoglycemic functional food or medicine.

关 键 词：芡种皮 多酚提取物 Α-葡萄糖苷酶 α-淀粉酶 抑制活性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]