藜麦总黄酮提取及大孔树脂纯化工艺的研究  

Study on extraction process of quinoa total flavonoids and purification process of macroporous resin

作　　者：董施彬[1,2] 宁亚萍[1,2] 杨喆[1,2] 张乔会[1,2] 董洁琼[1,2] 王丰俊[1,2] 王建中[1,2] 

机构地区：[1]北京林业大学生物科学与技术学院 [2]北京林业大学林业食品加工与安全北京市重点实验室,北京100083

出　　处：《食品工业科技》2015年第16期272-278,共7页Science and Technology of Food Industry

基　　金：林业公益行业科研专项资金(201004081)

摘　　要：目的:研究藜麦总黄酮的提取工艺与大孔树脂纯化工艺。方法:在单因素实验的基础上,通过正交实验法,研究藜麦总黄酮的最佳提取和纯化工艺。结果:最佳提取工艺条件为:超声时间25 min、温度90℃、料液比1∶10(g/m L)、回流时间为2 h、乙醇浓度90%。此条件下,藜麦总黄酮提取量为3.861 mg/g。盐析除蛋白,选择Na Cl添加量为15%。最佳大孔树脂纯化工艺为:p H2、流速2 BV/h、0.3 mg/m L的黄酮水溶液上样、8 BV水洗、丙酮洗脱。此条件下,纯化黄酮吸附率为80.91%。纯度可由初提物的7.12%提高到28.53%。Purpose.To study the extraction process of q uinoa flavonoids and purification process of macroporous resin. Method ：To study the optimal extraction and purification process of quinoa through orthogonal test based on single factor test. Results=The optimal extraction process conditions were：ultrasound time for 25 min, temperature 90 ℃,material and liquid ratio 1：10（g/mL）,refluxing time 2 h,ethanol concentration 90%. The total extracting amount of Quinoa flavonoids under these conditions was 3.861 mg/g. Perform salting-out to remove protein. Select NaCI additive amount as 15%. The optimal purification process of macroporous resin was：pH2, flow velocity 2 BV/h,0.3 mg/mL flavonoids aqueous solution,8 BV water washing,acetone elution. The purified flavonoids adsorption rate under these conditions was 80.91%. The purity could be improved from 7.12% of the original extracts to 28.53%.

关 键 词：藜麦 总黄酮 乙醇提取 大孔树脂纯化 

分 类 号：TS201.1[轻工技术与工程—食品科学]