Proportions of acetyl-histone-positive hepatocytes indicate the functional status and prognosis of cirrhotic patients  

作　　者：Ping Zhou Jie Xia Yong-Jie Zhou Jun Wan Li Li Ji Bao Yu-Jun Shi Hong Bu 

机构地区：[1]Laboratory of Pathology,West China Hospital,Sichuan University [2]Department of Pathology,West China Hospital,Sichuan University [3]Department of Pathology,Affiliated Hospital of North Sichuan Medical College

出　　处：《World Journal of Gastroenterology》2015年第21期6665-6674,共10页世界胃肠病学杂志（英文版）

基　　金：Supported by Natural Science Foundation of China,No.81170424

摘　　要：AIM:To investigate whether the proportions of acetylhistone-positive hepatocytes could be used as markers of deteriorating liver function.METHODS:In total,611 cirrhotic cases from 3701 patients who were diagnosed during the past 15 years were screened,and 152 follow-up cases were selected.Paraffin tissue microarray was prepared for immunohistochemistry to examine acetyl-histone expression.The proportions of positive hepatocytes were recorded,and their correlations to clinical and laboratory indicators were analyzed statistically.RESULTS:The proportions of H2AK5ac+,H3K9/K14ac+ and H3K27ac+ hepatocytes gradually increased with deteriorating liver function and with increasing levels of serum markers of liver injury.In the follow-up cases,patients with > 70% H2AK5ac+,H3K9/K14ac+ or H3K27ac+ hepatocytes had statistically lower survival rates(P < 0.05).Furthermore,> 70% H2AK5ac+ or H3K27ac+ hepatocytes were strong independent predictors of overall survival(P < 0.05).CONCLUSION:The proportions of acetyl-histonepositive hepatocytes are closely associated with the liver function and prognosis of cirrhotic patients.AIM: To investigate whether the proportions of acetylhistone- positive hepatocytes could be used as markers of deteriorating liver function. METHODS: In total, 611 cirrhotic cases from 3701 patients who were diagnosed during the past 15 years were screened, and 152 follow-up cases were selected. Paraffin tissue microarray was prepared for immunohistochemistry to examine acetyl-histone expression. The proportions of positive hepatocytes were recorded, and their correlations to clinical and laboratory indicators were analyzed statistically. RESULTS: The proportions of H2AK5ac(+), H3K9/K14ac(+) and H3K27ac(+) hepatocytes gradually increased with deteriorating liver function and with increasing levels of serum markers of liver injury. In the follow-up cases, patients with > 70% H2AK5ac(+), H3K9/K14ac(+) or H3K27ac(+) hepatocytes had statistically lower survival rates (P < 0.05). Furthermore, > 70% H2AK5ac(+) or H3K27ac(+) hepatocytes were strong independent predictors of overall survival (P < 0.05). CONCLUSION: The proportions of acetyl-histonepositive hepatocytes are closely associated with the liver function and prognosis of cirrhotic patients.

关 键 词：HISTONE ACETYLATION Cirrhosis Liver function PROGNOSIS 

分 类 号：R575.2[医药卫生—消化系统]