黄绿蜜环菌深层发酵工艺研究  

Studies on submerged fermentation technology of Armillaria luteo-rivens

作　　者：史强强[1,2] 党军[1] 苑祥[1,2] 文怀秀[1] 王启兰[1] 

机构地区：[1]中国科学院西北高原生物研究所中国科学院藏药研究重点实验室,西宁市新宁路23号810008 [2]中国科学院大学,北京市100049

出　　处：《工业微生物》2015年第4期35-38,共4页Industrial Microbiology

基　　金：青海省高新技术研究与发展计划(2012-G-218)

摘　　要：以人工驯化的黄绿蜜环菌菌种为材料,在优化的培养基组成及培养温度下进行培养。通过单因素实验,考察不同的摇瓶装量、搅拌速度、发酵时间和接种量对黄绿蜜环菌菌丝体产量的影响,在此基础上通过正交设计确定最优深层发酵工艺参数,并通过小试和中试对其进行验证,黄绿蜜环菌最优化发酵工艺参数:摇瓶装量为150 m L/500 m L、搅拌速度为125 r/min、发酵时间为10 d、接种量为15%,此工艺可应用于工业化生产。A domesticated strain of Armillaria luteo-rivens was cultivated under the conditions composition and culture temperature. Through the single factor experiment fermentation period and inoculum size on mycelia yield were investigated. optimum parameters of submerged fermentation were determined by using of optimized medium , the effects of medium volume, stirring rate, On the basis of single factor experiment, the orthogonal design, and then were verified on laboratory scale and pilot scale. The optimized parameters of fermentation were as follows： loading volume 150 mL/500 mL, stirring speed 125 r/min, fermentation period 10 d, inoculum size 15%. The result indicated that this technology could be applied to industrial production.

关 键 词：黄绿蜜环菌 深层发酵 发酵工艺 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]