产β-甘露聚糖酶菌株的筛选鉴定及产酶条件的优化  

Screening and identification of β-mannanase producing strains and optimization of fermentation conditions

作　　者：杨苗[1] 卢晓华[1] 王常高[1] 杜馨[1] 林建国[1] 蔡俊[1] 

机构地区：[1]湖北工业大学发酵工程教育部重点实验室和工业发酵湖北省协同创新中心,湖北武汉430068

出　　处：《工业微生物》2015年第4期51-57,共7页Industrial Microbiology

摘　　要：利用刚果红染色法从土壤中筛选到一株产β-甘露聚糖酶的菌株MY271,该菌株经形态学、生理生化及系统发育学方法鉴定为路德维希肠杆菌(Enterobacter ludwigii)。该菌株在初始条件下培养48 h,发酵上清液中β-甘露聚糖酶酶活可达2.87 U/m L。利用单因素试验对该菌产酶发酵条件进行优化以提高酶活,优化所得最佳发酵条件为:接种量9%,装液量50 m L/250 m L,初始p H7.0,发酵温度31℃,发酵周期48 h。最佳碳源为魔芋精粉(添加量0.8%),最佳氮源为蛋白胨(添加量1.9%)。在最佳条件下发酵48 h,发酵上清液中β-甘露聚糖酶活提升到38.42 U/m L,是优化前的13.4倍。Using congo red method, strains for producing β-mannanase were isolated from soil. After screening, a strain was identified as Enterobacter ludwigii through morphological, physiological and biochemical characteristics combined with phylogenetic factor test to 9% , loading analysis and then was named as MY271. The fermentation conditions for this stain were optimized by single- improve its β-mannanase producing level. The optimized conditions obtained were as follows： inoculum size volume 50 mL/250 mL, initial pH 7.0, fermentation temperature 31℃, fermentation period 48 h, konjac powder 0.8% and peptone 1.9%. Under the optimized conditions, the β-mannanase activity in fermentation supematant was as high as 38.42 U/mL, which was 13.4 fold than that before optimization.

关 键 词：Β-甘露聚糖酶 筛选 鉴定 发酵条件优化 

分 类 号：TQ925[轻工技术与工程—发酵工程]