利用电子自旋共振技术研究黄海海燕体壁多糖的体外抗氧化活性  

Study on in vitro antioxidant activity of starfish Asterina pectinifera bodywall polysaccharide using electron spin

作　　者：刘山[1,2] 李楠[1,2] 傅卉[1,2] 许喆[1] 常思佳[1] 秦磊[1,2] 董秀萍[1,2] 启航[1,2] 

机构地区：[1]大连工业大学食品学院,辽宁大连116034 [2]国家海洋食品工程技术研究中心,辽宁大连116034

出　　处：《食品与机械》2015年第4期155-158,共4页Food and Machinery

基　　金：国家高技术研究发展计划(编号:2014AA093602);辽宁省农业领域青年科技创新人才培养计划(编号:2014001);国家海洋食品工程技术研究中心立项建设项目(编号:2012FU125X03)

摘　　要：以新鲜黄海海燕体壁为原料,制备粗多糖及精多糖。采用电子自旋共振波谱技术(ESR),检测不同浓度的黄海海燕体壁粗多糖及精多糖对羟基自由基、DPPH和超氧阴离子的清除能力。结果表明:黄海海燕体壁粗多糖及精多糖对羟基自由基、DPPH和超氧阴离子的清除能力随浓度升高而增强,黄海海燕体壁粗多糖对3种自由基清除能力的IC50值分别为59.56,27.29,32.36mg/mL;精多糖对3种自由基清除能力的IC50值分别为44.96,13.97,30.51mg/mL。在相同多糖干粉浓度下,黄海海燕体壁精多糖对羟基自由基、DPPH和超氧阴离子的清除能力强于黄海海燕体壁粗多糖。The raw polysaccharide and refined polysaccharide were prepared from the body wall of fresh starfish Asterina pectinifera. The scavenging capacity of the raw polysaccharide and refined poly- saccharide on hydroxyl radical, DPPH radical and superoxide anion were detected using electron spin resonance （ESR）. The result indi- cated that the polysaccharide from the body wall of fresh starfish As-terina pectinifera possessed a certain scavenging capacity. And this scavenging capacity was enhanced with concentration rising. The IC50 values of three radical scavenging activities of raw polysaccharide were 59.56,27.29,31.36 mg/mL and the IC50 values of three radical scavenging activities of refined polysaccharide are 44. 96, 13. 97, 30. 51 mg/mL. At the same concentration of poly＇saceharide powder, the scavenging capacities of refined polysaccharidewere stronger than the raw polysaccharide for hydroxyl radical, DPPH and superoxide anion scavenging abilities.

关 键 词：黄海海燕 多糖 自由基 抗氧化活性 电子自旋共振波谱技术 

分 类 号：TS201.23[轻工技术与工程—食品科学]