HPLC法快速测定乳粉中VC棕榈酸酯的含量  

Rapid Determination of Content of Ascorbyl Palmitate in Milk Powder by HPLC

作　　者：杜淑霞[1] 徐丽[1] 廖延智[1] 司徒满泉[1] 李文清[2] 

机构地区：[1]广东轻工职业技术学院,广东广州510300 [2]暨南大学,广东广州510632

出　　处：《食品研究与开发》2015年第13期84-86,共3页Food Research and Development

基　　金：广东省科技计划项目"安全;高效食品添加剂的开发"(2010A080403001);广东省产学研基地滚动项目"酶法合成L-抗坏血酸棕榈酸酯的中试研究"(2013CXZDC006)

摘　　要：建立了利用高效液相色谱仪快速测定乳粉中Vc棕榈酸酯含量的方法。以含0.1%草酸的乙醇溶液为提取剂,以甲醇和0.15%草酸(体积比为90∶10)为流动相,经Inertsil ODS-SP色谱柱分离,采用二极管阵列检测器,在245 nm波长下进行测定。VC棕榈酸酯的质量浓度在0.5μg/m L^100μg/m L浓度范围内与峰面积呈良好的线性关系。以3倍基线噪声所对应的浓度计算VC棕榈酸酯的检出限为2μg/g。加标回收率在92.01%~105.84%之间,相对标准偏差(n=6)在1.02%~2.70%之间。The method for rapid determination of the content of ascorbyl palmitate in milk powder by high performance liquid chromatograph （HPLC） was established. It was separated through Inertsil ODS-SP chromatographic column with ethanol containing 0.1%oxalic acid as extraction agent and with methanol and 0.15%oxalic acid （ vol/vol=90∶10） as mobile phase, and then was measured under 245 nm wavelength with a diode array detector. There was a good linear relationship between mass concentration of ascorbyl palmitate and peak area when the mass concentration was from 0.5 μg/mL to 100 μg/mL. Detection limit of ascorbyl palmitate was calculated as 2μg/g by the concentration which corresponded to 3 times baseline noise. Standard addition recovery was 92.01%-105.84%, and relative standard deviation （n=6） was 1.02%-2.70%.

关 键 词：VC棕榈酸酯 高效液相色谱法(HPLC) 乳粉 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]