过表达Vav1蛋白对乳腺癌细胞系T47D细胞增殖的影响  

Effects of Vav1Overexpression on Cell Proliferation in Human Breast Cancer Cell Line T47D

作　　者：杜明娟[1] 陈祥东[1] 周晓丽[1] 张翠竹[1] 

机构地区：[1]南开大学生命科学学院药物化学生物学国家重点实验室,天津300071

出　　处：《南开大学学报（自然科学版）》2015年第4期37-42,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：Vav1是造血系统中的重要蛋白,具有鸟苷酸交换因子(guanine nucleotide exchange factor,GEF)活性,能够催化Rac/Rho GTP酶的活性,从而参与众多的细胞事件,如细胞骨架重组,基因的转录,免疫细胞的发育和活化,细胞增殖和凋亡等.近年来,在包括乳腺癌在内的人类癌症中均发现有Vav1的表达,但对于Vav1在乳腺癌细胞中的具体功能和机制仍不清楚.本研究中,通过慢病毒包装系统感染乳腺癌细胞系T47D构建了稳定表达Vav1的乳腺癌细胞系,通过WST-1检测细胞增殖和细胞流式法检测细胞周期发现Vav1能够促进细胞增殖,同时通过蛋白免疫印迹技术和报告基因系统发现这一过程可能跟Vav1增强转录因子NF-κB转录活性上调细胞周期相关蛋白Cyclin D1相关.Vavl, a guanine nucleotide exchange factor （GEF） for Rho family GTPases, is hematopoietic protein involved in a variety of cellular events. In recent years, Vavl has been reported to aberrantly express in several human malignancies including human breast cancer. But the specific function and mechanisms of Vavl in breast cancer is not clear. In this study, a stable line expressing Vavl was established with lentivirus system in human breast cancer cell line T47D. Function studies indicated Vavl overexpression might increase NF-rB tran- scription to up-regulated cell cycle-related protein, Cyclin D1, and consequently enhance cell proliferation in T47D cells. The results suggested ectopic expression of Vavl in breast cancer may play a significant role in tu- morigenesis.

关 键 词：乳腺癌 T47D 细胞增殖 CYCLIN D1 NF-ΚB 

分 类 号：R737.9[医药卫生—肿瘤]