高效分解水产原料的菌株分离鉴定和组合发酵初探  

ISOLATION AND IDENTIFICATION OF STRAINS WHICH HAVE HIGH EFFICIENT DECOMPOSITION ON LOW-VALUE AQUATIC PRODUCTS AND EXPLORATION OF COMBINATION FERMENTATION

作　　者：宋敏[1] 刘为[1] 张鹰炯 童晓倩 李鹏[1] 王斌[1] 罗红宇[1] 

机构地区：[1]浙江省海产品健康危害因素关键技术研究重点实验室浙江海洋学院食品与医药学院,舟山316022 [2]岱山县绿康源海洋生物技术有限公司,舟山316200

出　　处：《海洋与湖沼》2015年第4期800-807,共8页Oceanologia Et Limnologia Sinica

基　　金：海洋公益性行业科研专项经费资助;201305016号

摘　　要：为从一种组成未知的商用复合菌中分离并筛选出优势发酵菌株,本实验采用稀释平板培养法,对菌种进行分离纯化,同时参照《真菌鉴定手册》和《常见细菌鉴定手册》,根据其形态学特征、生理生化特性,并分别结合16S r DNA和18S r DNA序列的比对分析对菌种进行鉴定。另外,本研究通过生长曲线测定,单菌种发酵试验以及多菌种混合发酵发酵试验,以游离氨基酸态氮为指标,以期筛选出发酵低值水产品制备菌肥的最佳菌株组合。结果显示,共分离得到4株菌,分别为a热带假丝酵母、b罗伦隐球菌、c枯草芽孢杆菌、d蜡样芽胞杆菌。且菌种组合acd发酵后游离氨基酸态氮的含量较对照组上升最显著,为8.276g/L。故可确定最佳发酵菌株配方为acd(热带假丝酵母+枯草芽孢杆菌+蜡样芽胞杆菌)。In order to screen strains with high efficient decomposition of low-value aquatic products from an unknown compound strains. We isolated and purified strains on the low-value aquatic products culture medium by the dilution plate culture method. According to the characteristics of morphology, physiology and biochemistry tests and the comparison of 16 S r DNA, 18 S r DNA sequence, the strains were classified. In addition, in order to screen good fermentation strains, we did the determination of growth curve, single strain fermentation test and mixed strains fermentation test. Four strains were isolated and they were named a, b, c, d respectively. The strain a was classified into Candida tropicalis, the strain b was classified into Cryptococcus laurentii, the strain c was classified into Bacillus subtilis, the strain d was classified into Bacillus cereus. And the optimal fermentation strains combination was acd（Candida tropicalis ＋ Bacillus subtilis ＋ Bacillus cereus）.

关 键 词：低值水产品 分离鉴定 发酵 游离氨基酸态氮 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]