海藻胶低聚寡糖的化学氧化制备纯化技术及保水理化性质分析  

ALGINATE OLIGOSACCHARIDES CHEMICAL OXIDATION PREPARATION AND PURIFICATION TECHNOLOGY AND THE PHYSICAL AND CHEMICAL PROPERTIES ANALYSIS

作　　者：李海波[1] 谢超[2] 丁晨[2] 黄菊[2] 裘晓华[2] 林琳[2] 

机构地区：[1]浙江国际海运职业技术学院,舟山316021 [2]浙江省海产品健康危害因素关键技术研究重点实验室浙江海洋学院食品与医药学院,舟山316022

出　　处：《海洋与湖沼》2015年第4期915-921,共7页Oceanologia Et Limnologia Sinica

基　　金：浙江省自然科学基金项目;LY14C200002号;舟山市科技计划项目;2013C11007号;2014C41011号;2014C31049号;浙江省重大科技项目;2013C02023-2号

摘　　要：海藻胶低聚寡糖是从马尾藻等藻类中提取出来的一种多糖类物质,具有抗氧化及保水等生物活性。本文采用氧化降解技术对海藻胶低聚寡糖的制备工艺进行了研究,通过对氧化过程中氧化剂浓度、反应温度、反应时间等进行研究,结果表明:当氧化剂H2O2添加浓度控制在6%左右,温度控制在60°C,时间控制在8h时,海藻胶降解为低聚寡糖的产率最高,达到60%左右。进一步对海藻胶低聚寡糖进行纯化,结果表明当温度控制在27°C,时间在2h时,分子量为6000—8000Da海藻胶低聚寡糖分离效率最高,达到70%以上,并对6000—8000Da海藻胶低聚寡糖的保水理化性能进行分析,结果表明6000—8000Da海藻胶低聚寡糖具有良好的保水性能。本研究可为开发一种安全、高效、节能、环保,适用于鱿鱼和虾仁等的生物保水剂提供理论基础。Alginate oligosaccharide is derived .from seaweed gel degradation sugars, acidity of the oceans has bacteriostatic, enhance the body＇s immune function, antioxidant with biological activity such as water, with endogenous sugar chain structure of many of the similarities, has wide development prospects. In this paper, the oxidative degradation technology of alginate oligosaccharide preparation process was studied, and the chemical oxidation method in the process, with H2O2 as oxidant, under acid condition, the four factors three levels orthogonal experiments, the preparation of oligosaccharide in the best conditions of molecular weight below 8000, when the concentration of H2O2 add control at about 6%, the temperature control at 60℃, time control in 8h, alginate oligosaccharide best production rate of degradation of oligosaccharides, around 60%; in seaweed gel preparation process, the separation of oligosaccharides with n-butanol/formic acid/water = 4/6/1 （F/F/F） as eluent, the samples using silica gel column to secondary separation, mass spectrometry analysis results show that the polymerization degree of 1000--8000 Da oligosaccharide basic get separated, of which 4000--6000 Da, 6000--8000 Da low purity oligosaccharide is bigger. The study for a better development and utilization of algae rubber resources, promoting the development of Marine biological products in China is of great significance.

关 键 词：海藻胶低聚寡糖 氧化降解技术 纯化技术 保水功能 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]