玉米样品前处理方法和掩蔽剂对ELISA检测玉米赤霉烯酮的影响  

Effect of Pretreatment Methods and Masking Agents for on the Detection of Zearalenone in Corn by ELISA

作　　者：甄玉萍 裴世春[1] 王岩[1] 高建伟[1] 李妍[1] 

机构地区：[1]齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔161006

出　　处：《食品科学》2015年第16期255-260,共6页Food Science

基　　金：齐齐哈尔大学研究生创新科研项目(YJSCX2014-015X);国家科技基础性工作专项(2013FY113400);黑龙江省教育厅科学计划项目(12541871)

摘　　要：探讨粉碎时间、提取剂、掩蔽剂质量分数等因素对酶联免疫吸附分析(enzyme-linked immunosorbent assay,ELISA)法检测玉米赤霉烯酮的影响。结果表明,玉米样品粉碎时间180 s以上、70%甲醇溶液为提取剂、添加0.025%鱼皮胶的磷酸盐缓冲液为掩蔽剂时,检测结果最为稳定。本实验结果对高稳定性玉米赤霉烯酮检测ELISA试剂盒的开发具有参考价值。In the present study, the effects of grinding time, extraction solvents and different amounts of masking agents on the detection of zearalenone were explored. The most stable results were obtained when corn samples were ground for more than 180 s, and then extracted with 70% methanol using phosphate buffer solution with 0.025% fish skin gelatin as the masking agent. The findings of this study can provide a reference for the development of highly stable ELISA kit for the detection of zearalenone in corn.

关 键 词：真菌毒素 玉米赤霉烯酮 酶联免疫吸附分析法 定量检测 

分 类 号：R446.61[医药卫生—诊断学]