保健食品双歧杆菌和乳酸菌计数中4种稀释液的比较  

Comparison of 4 diluents for enumeration of Bifidobacteria and lactic acid bacteria in health food

作　　者：王似锦[1] 江志杰[1] 牛振东[1] 张光华[1] 高春[1] 刘文杰[1] 

机构地区：[1]北京市药品检验所,北京100035

出　　处：《中国卫生检验杂志》2015年第16期2700-2702,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的选出益生菌类保健食品双歧杆菌和乳酸菌计数中最优稀释液。方法对2个品种4个批次的益生菌类保健食品,分别采用4种稀释液:生理盐水(NS)、林格液(QSR液)、磷酸盐缓冲液(PBS缓冲液)、半胱氨酸磷酸盐缓冲液(CYS缓冲液)制备样品匀液,进行双歧杆菌和乳酸菌计数,并比较结果。结果 4种稀释液中,使用CYS缓冲液能够得到最高的计数结果。CYS缓冲液有更强的缓冲能力,能够提供更接近中性的p H值;可以提供有利于双歧杆菌存活的厌氧环境;可以使双歧杆菌和乳酸菌更充分的释放和分散均匀。结论 CYS缓冲液为最优稀释液。Objective To select optimum diluent for enumeration of Bifidobacteria and lactic acid bacteria in probiotic health food. Methods 4 diluents（ NS,QSR,PBS and CYS） were used to prepare initial suspension and dilutions in 2 kinds of health food（ 4 Lots）,and the results of enumeration of Bifidobacteria and lactic acid bacteria were compared. Results CYS-buffer yielded highest counts in the 4 diluents. The buffer capacity of CYS- buffer is stronger,and it can provide near neutral p H value. CYS- buffer can provide anaerobic environment which is beneficial to the survival of Bifidobacteria. When CYS-buffer is used,the neutral p H and surface active agent can make the Bifidobacteria and lactic acid bacteria more fully released and dispersed. Conclusion CYS- buffer is the best diluent.

关 键 词：益生菌 保健食品 双歧杆菌 乳酸菌 稀释液 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]