苹果中展青霉素的气相色谱-质谱检测研究  

Detetmination of Patulin in Apple Fruit by Gas Chromatography-Mass Spectrometry Analysis

作　　者：毛艳玲[1] 蔡艳[1] 罗昊轩 杨震峰[1] 饶志恒[1] 王唯正 

机构地区：[1]浙江万里学院生物与环境学院,浙江宁波315100

出　　处：《核农学报》2015年第9期1757-1765,共9页Journal of Nuclear Agricultural Sciences

基　　金：浙江省公益性技术应用研究计划项目(2013C37097);国家大学生创新创业训练项目(201310876008;201510876002);浙江省"生物工程"重中之重学科自设课题(ZS2015001)

摘　　要：为研究苹果腐烂过程中展青霉素的积累和迁移,采用液液萃取、原创Qu ECh ERS法、AOAC.2007法、EN15662法4种前处理技术结合气相色谱-质谱联用法测定苹果中展青霉素,对提取溶剂、衍生化试剂、衍生化条件、不同检测模式等条件进行了优化;采用空白基质配制标准溶液进行补偿和消除,并对不同腐烂程度的苹果样品中的腐烂和未腐烂部分进行展青霉素检测。结果表明,AOAC.2007和EN15662法较其他方法具有回收率高（92.41%-99.09%）、相对标准偏差小（2.01%-5.87%）、操作简单、溶剂用量少等优点,该方法快速、高灵敏、准确,适合对苹果中的PAT进行确证和定量测定;苹果的腐烂率和展青霉素的含量之间没有正相关性,腐烂部分和未腐烂部分展青霉素的含量分别为0.147-40.808μg·g^-1和0.0016-1.254μg·g^-1,表明苹果中展青霉素会从腐烂区域向未腐烂区域迁移,对人体存在潜在危险。本研究通过建立苹果中PAT高灵敏度检测方法和研究采后苹果中PAT的迁移规律,为苹果贮运和质量安全控制提供重要的依据。In order to establish detection method and study the accumulation and migration of patulin,liquid-liquid extraction and Qu ECh ERS extraction（ original Qu ECh ERS method,AOAC. 2007 method,EN15662 method） coupled with gas chromatography-mass spectrometry were developed to determine patulin in decaying of apple fruits. The solvent selection,derivatization reagents,derivatization conditions and different detection modes were optimized,and calibration was performed with matrix-matched standard solutions. The results showed that AOAC. 2007 and EN15662 methods had superiority in patulin detection with higher detection recovery（92. 41% to 99. 09%）,lower relative standard deviation（2. 01% to 5. 87%）,and less solvent,high sensitivity and accuracy. The results also showed that there is no correlation between the decay rate and patulin contents in apples. The patulin values of decayed parts and undecayed parts range from 0. 147 to 40. 808μg·g^- 1and 0. 0016 to 1. 254μg·g^- 1,respectively,suggesting that patulin might migrate to health tissue from rotten areas. The high sensitivity detection method for PAT in apples was established and migration of PAT in postharvest apples was studied,laying theoretical and practical basis for the storage,transportation,quality and safety control of apples.

关 键 词：展青霉素 气相色谱-质谱 QU ECH ERS法 基质效应 苹果 

分 类 号：S661.1[农业科学—果树学] O657.63[农业科学—园艺学]