嗜热真菌Talaromyces thermophilus F1208产木聚糖酶条件优化  

Optimization of High-producing Xylanase by Thermophilic Fungi Talaromyces thermophilus F1208

作　　者：滕超[1,2] 范园园[1] 曲玲玉 查沛娜[1] 肖林 李秀婷[1,2] 

机构地区：[1]食品营养与人类健康北京高精尖创新中心(北京工商大学),北京100048 [2]北京市食品添加剂工程技术研究中心(北京工商大学),北京100048 [3]山东龙力生物科技股份有限公司,山东禹城251022

出　　处：《中国食品学报》2015年第8期85-93,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然基金-青年科学基金项目(No.31201449);国家高技术研究发展(863)计划项目(2012AA021502)

摘　　要：对木聚糖酶嗜热真菌嗜热踝节菌F1208的产酶条件进行优化，经单因素试验及Box—Behnken响应面分析，确定该菌液体发酵培养基，其组成是：碳源为玉米芯粉，碳源粒度24—40目，碳源质量分数5％；氮源为大豆蛋白胨与酵母膏复合氮源（质量比l：3），氮源质量分数2％；辅助添加物甘油（0．2％）；培养基初始pH6．4。优化后最佳产酶条件是：装液量60mL，培养温度48℃，摇床转速200r／min。在此条件下发酵培养7d，产酶量迭3026．65U，mL，是初始酶活（960．39U／mL）的3．15倍，为同类研究中较高水平，具有较好的应用前景。The culture conditions of a thermophilic fungus F1208 with high yield of xylanase activity were optimizedby single-factor experiment and Box-Behnken design matrix of response surface methodology. The influences of medium composition and fermentation conditions in the cultivation were investigated. A high xylanase activity of 3 026.65 U/mL was obtained in 7 days, which was 3.15 fold of the initial enzyme activity （960.39 U/mL）. The results indicated optimum conditions were corncob, mesh number 24-40, 5%; soya peptone and yeast cream （ratio 1：3） 2%; glycerine 0.2%; pH 6.4, and the fermentation conditions was 60 mL, 48℃ and 200 r/min.

关 键 词：木聚糖酶 嗜热踝节菌 响应面分析 优化 

分 类 号：TQ925[轻工技术与工程—发酵工程]