响应面法优化眼点拟微绿球藻酶法提油工艺研究  

Optimization of enzymatic extraction of oil from Nannochloropsis oculata by response surface methodology

作　　者：陆向红[1] 贾俊乾[1] 张秋红[1] 黄晨蕾 计建炳[1] 

机构地区：[1]浙江工业大学化学工程学院,浙江省生物质燃料利用技术研究重点实验室,杭州310014

出　　处：《中国油脂》2015年第9期13-17,共5页China Oils and Fats

基　　金：国家高技术研究发展计划(863)资助(2014AA022103)

摘　　要：采用纤维素酶酶解眼点拟微绿球藻提取胞内油脂。在单因素实验的基础上,以酶用量、酶解时间、酶解温度、酶解p H为自变量,油脂提取效率和EPA提取效率为响应值进行响应面法优化酶解反应条件。结果表明:对油脂提取效果影响的主次顺序为酶解温度>酶解p H>酶用量>酶解时间,在酶用量1.59 g/kg(以干藻粉质量计)、酶解时间2.11 h、酶解温度39.3℃、酶解p H 4.17的条件下,油脂提取效率达到最大值98.72%#0.15%;对EPA提取效果影响的主次顺序为酶解温度>酶解时间>酶解p H>酶用量,在酶用量1.60 g/kg(以干藻粉质量计)、酶解时间1.86 h、酶解温度37.9℃、酶解p H 4.10的条件下,EPA提取效率达到最大值97.86%#0.12%。The enzymatic extraction of oil from Nannochloropsis oculata by cellulase was studied. Using enzyme dosage, enzymolysis time, enzymolysis temperature and enzymolysis pH as variables and extraction efficiencies of oil and EPA as response values, the enzymatic extraction conditions were optimized by re- sponse surface methodology on the basis of single factor experiment. The results showed that enzymolysis temperature had the greatest effect on the extraction efficiency of oil, followed by enzymolysis pH, enzyme dosage and enzymolysis time, and extraction efficiency of oil reached 98.72% ＋0.15% under the condi- tions of enzyme dosage I. 59 g/kg （based on the mass of dry algal powder）, enzymolysis time 2.11 h, enzymolysis temperature 39.3 ℃ and enzymolysis pH 4.17. Enzymolysis temperature had the largest impact on the extraction efficiency of EPA, followed by enzymolysis time, enzymolysis pH and enzyme dosage, and the extraction efficiency of EPA was 97.86% ±0.12% under the conditions of enzyme dosage 1.60 g/kg（based on the mass of dry algal powder）, enzymolysis time 1.86 h, enzymolysis temperature 37.9℃ and enzymolysis pH 4.10.

关 键 词：眼点拟微绿球藻 纤维素酶 酶法提取 油脂 EPA 

分 类 号：TQ644[化学工程—精细化工]