采后硅酸钠处理对厚皮甜瓜防卫基因mRNA的诱导表达  

Postharvest sodium silicate treatment induced mRNA expression of defense genes in Muskmelon Fruit

作　　者：魏娟[1] 张溪桐[1] 毕阳[1] 王毅[1] 董柏余 王婷[1] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070

出　　处：《食品工业科技》2015年第19期163-165,170,共4页Science and Technology of Food Industry

基　　金：采后硅酸钠处理诱导的厚皮甜瓜抑制消减文库构建及EST序列分析(甘肃省青年科技基金计划1208RTYA095)

摘　　要：本文研究了采后100 mmol/L硅酸钠浸泡处理对甜瓜果实苯丙烷代谢途径关键酶苯丙氨酸解氨酶(PAL)、查尔酮合成酶(CHS)、及抗病相关酶过氧化物酶(POD)、β-1,3-葡聚糖酶基因(β-1,3-Glu)mRNA表达的影响。结果表明:硅酸钠处理可提高PAL、CHS、POD、β-1,3葡聚糖酶基因mRNA的表达。其中经硅酸钠处理后PAL、CHS、POD基因分别于6、6、2 d达到最大表达量,其表达量分别为对照组的2.69、2.48、3.77倍。由此表明,采后硅酸钠处理厚皮甜瓜果实可通过诱导苯丙烷代谢途径关键酶PAL、CHS,及抗病相关酶POD、β-1,3葡聚糖酶基因的转录增强采后抗病性。In this paper,the effect of postharvest sodium silicate dipping at 100 mmol / L on Phenylalanine ammonialyase（ PAL）,Chalcone synthase（ CHS）,Peroxidase（ POD）,β- 1,3- glucanase（ β- 1,3- Glu） mRNA expression of muskmelon fruit（ Cucumis melo L.） were evaluated in this experiments. The results indicated that the mRNA expression of PAL,CHS,POD and β- 1,3- Glu genes were increased by the sodium silicate. PAL,CHS and POD genes with sodium silicate treatment expressed maximum after 6,6,2 d respectively,the expression of PAL,CHS and POD were 2.69,2.48,3.77 times that of the control.It was suggested that postharvest sodium silicate treatment enhanced the disease resistance by inducing the mRNA expression of PAL,CHS,POD and β-1,3- Glu genes in muskmelon fruits.

关 键 词：厚皮甜瓜 硅酸钠 实时定量PCR 防卫基因 诱导抗性 

分 类 号：TS201.1[轻工技术与工程—食品科学]