超声法提取香料烟花蕾多糖及其抗氧化活性  

Ultrasound-assisted Extraction and Antioxidative Activities of Polysaccharides from Oriental Tobacco Buds

作　　者：刘绍华[1] 刘远上 孙斯文[2] 胡志忠[1] 肖源[2,3] 田兆福[1] 许春平[2] 

机构地区：[1]广西中烟工业有限责任公司技术中心,南宁市北湖南路28号530001 [2]郑州轻工业学院食品与生物工程学院 [3]红云红河有限责任公司新疆卷烟厂

出　　处：《烟草科技》2015年第8期25-30,共6页Tobacco Science & Technology

摘　　要：采用正交实验法优化了超声波法提取香料烟花蕾多糖的工艺条件;利用琼脂糖CL-6B层析法对粗多糖进行分离纯化,得到两个组分(Fr-I和Fr-II);分别采用GC(气相色谱法)、FT-IR(傅里叶变换红外光谱法)和TGA(热重分析法)法分析了Fr-I和Fr-II的组成、结构特征和热稳定性;测定了两个组分清除羟基自由基和DPPH自由基的能力。结果表明:1超声波法提取香料烟花蕾多糖的最佳工艺条件为超声功率300 W,液料比30∶1(m L/g),70℃提取6 min;此条件下多糖的提取率为2.62%。2Fr-I和Fr-II的分子量分别为181.97和16.27 k Da。3Fr-I和Fr-II两个组分均为杂多糖,其IR光谱显示分别具有α-构型和β-构型。4TGA结果显示Fr-I和Fr-II的降解温度(Td)分别为195.7和180.5℃。5两个组分均对羟基自由基和DPPH自由基有良好的清除能力;Fr-II比Fr-I的抗氧化能力更强。The technological parameters of ultrasound-assisted extraction of polysaccharides from oriental tobacco buds were optimized with orthogonal test. The crude polysaccharide product was fractionated and purified with a sepharose CL-6B chromatographic column and two polysaccharide fractions （Fr-I and Fr-II） were obtained. The chemical compositions, structural characters and thermal stabilities of Fr-I and Fr-II were analyzed by GC, FT-IR and TGA, and their abilities for scavenging hydroxyl radical and DPPH radical were also determined. The results showed that： 1） The optimized conditions for extracting polysaccharides from oriental tobacco buds were ultrasound power 300 W, ratio of water to raw material 30∶1 （mL/g）, extracting for 6 min at 70 ℃. The yield of polysaccharide extracted under above conditions was 2.62 %. 2） The molecular weights of Fr-I and Fr-II were 181.97 and 16.27 kDa, respectively. 3） Both Fr-I and Fr-II were heteropolysaccharides, IR spectra revealed that they were of α- and β-configurations,respectively. 4） TGA results indicated that the degradation temperatures （Td） of Fr-I and Fr-II were 195.7 and 180.5 ℃, respectively. 5） Both Fr-I and Fr-II possessed good abilities for scavenging hydroxyl radical and DPPH radical, and the antioxidative activity of Fr-II was higher than that of Fr-I.

关 键 词：香料烟 花蕾 超声辅助提取 多糖 抗氧化活性 

分 类 号：TS49[农业科学—烟草工业]