双水相木聚糖酶水解体系制备木二糖及其纯化  

Preparation of Xylobiose by Xylanase Hydrolysis in Aqueous Two-phase System and Its Purification

作　　者：李鑫[1] 顾夕梅 绳敖楠 张丽[1] 陈晓佩[1] 勇强[1] 

机构地区：[1]南京林业大学化学工程学院,江苏南京210037

出　　处：《林产化学与工业》2015年第5期93-97,共5页Chemistry and Industry of Forest Products

基　　金：江苏省高校自然科学研究重大项目(14KJA220003);江苏省自然科学基金项目(BK20131426);江苏高校优势学科建设工程项目资助项目(无编号)

摘　　要：采用聚乙二醇6000/柠檬酸钠的双水相木聚糖酶水解体系制备木二糖,以10 g/L桦木木聚糖为底物,木聚糖酶的酶用量为20 IU/g(以木聚糖质量计),在50℃、p H值5.6条件下,水解8 h制备木二糖,木二糖的酶解得率为13.8%。双水相水解体系对木聚糖酶回收率为66.4%,对木二糖回收率为87.2%;采用凝胶层析纯化方法,以葡聚糖凝胶LH-20为分离介质,脱气超纯水为洗脱剂,在柱温35℃、洗脱溶剂流速0.1 m L/min、上样量0.3 m L条件下,分离纯化木二糖,木二糖纯度为98.5%。Xylobiose was prepared by xylanase hydrolysis of birchwood xylan in the PEG6000 / sodium citrate aqueous two-phase system（ ATPS）. The optimized conditions for enzymatic hydrolysis in ATPS were birchwood xylan 10 g / L,xylanase 20 IU / g（ calculated by the mass of xylan）,50 ℃ and p H 5. 6. The enzymatic hydrolysis yield of xylobiose was 13. 8% for 8h. The recoveries of xylanase and xylobiose were 66. 4% and 87. 2%,respectively. Then,purification of xylobiose was carried out on Sephadex LH-20 with using degassed high purified water as eluent. The optimized conditions for purification of xylobiose were column temperature 35 ℃,0. 1 m L / min and sample volume 0. 3 m L. After purification,the purity of xylobiose was improved to98. 5% under the optimized conditions.

关 键 词：木聚糖酶 双水相体系 酶水解 凝胶层析 木二糖 

分 类 号：TQ35[化学工程] Q556[生物学—生物化学]