Platycodon grandiflorum （ Jacq. ） A. mey extracts inhibits hepatic fibrosis DC. and Panax Ginseng C. A. in activated hepatic stellate cells  

出　　处：《中国药理学通报》2015年第B11期142-143,共2页Chinese Pharmacological Bulletin

摘　　要：Fibrosis is a phenomenon of fibrous connective tissue increase in the organs. Activated hepatic stellate cells （HSCs） are the preferred targets of anti-hepatic fibrotic therapies. Ginseng and Platycodon grandiflorum are impor- tant traditional Chinese medicine herbs. The study was to investigate the anti-fibrosis effects of ginseng extract and Platycodon grandiflorum extract in HSCs. Extract include Ginseng water extract and alcohol extract, Platycodon gran- diflorum water extract and alcohol extract, combination of both water extract and alcohol extract. Activated hepatic stellate cells were treated with Ginseng and Platycodon grandiflorum extracts for 24h. The maximum concentration of the drug is 2000 mg · L^-1, the lowest concentration of 7.8 mg · L^-1 , with concentration gradient dilution. 3-（4 , 5- dimethylthiazol-2-yl）-2, 5-diphenylte-trazolium bromide （MTT） can evaluate the survival rate of cells. The expres- sions of SIRT1, NF-KB and p53 were detected by western blot. The results showed that extract effectively decreased the HSC cells viability in concentration dependent manner. Among them, the effect of combination of both extract is best. Ginseng and Platycodon grandiflorum activated the protein expressions of SIRT1 in activated HSCs. The expres- sion of NF-KB and p53 were significantly decreased by Ginseng and Platycodon grandiflorum. This study demonstrated that ginseng extract and Platycodon grandiflorum extract showed obviously anti-fibrosis effect, and decreased expres-sion of NF-KB and p53 via SIRT1 signaling pathway.

关 键 词：HEPATIC FIBROSIS GINSENG Platycodon grandiflorum HSCS SIRT1 p53 

分 类 号：R[医药卫生]