降亚硝酸盐短乳杆菌的诱变选育研究  

Mutagenesis breeding research of Lactobacillus brevis of nitrite reduction

作　　者：李泽丽[1] 周月[2] 龚钢明[2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海应用技术学院香料香精技术与工程学院,上海201418

出　　处：《上海师范大学学报（自然科学版）》2015年第5期495-499,共5页Journal of Shanghai Normal University(Natural Sciences)

摘　　要：食品中亚硝酸盐的污染问题一直是食品安全问题的焦点之一,利用生物技术方法降解食品中的亚硝酸盐成为热点.以实验室保存的一株具有降解亚硝酸盐能力的短乳杆菌(Lactobacillus brevis)C2为出发菌株,经过15 W、254 nm、20 cm处紫外诱变(UV)120 s及0.8%的硫酸二乙酯(DES)于37℃诱变40 min的复合诱变,进行筛选,获得高效降解亚硝酸盐菌株UV6-DS2,相对于出发菌株,在400 mg/L亚硝酸盐的环境下,经12 h降解后,亚硝酸盐的降解率由92.8%提高到97.8%,探究其于食品中的应用可以有效地降低食品中亚硝酸盐的含量.The pollution of nitrite in food became one of the focus of food safety issues, the use of biotechnology methods degrad- ing nitrite became hotspot. The primitive strain was Lactobacillus brevis C2, preserved in our laboratory, had the ability to degrade nitrite, through composite mutagenesis of 15 W,254 nm,20 cm ultraviolet mutagenesis （UV） for 120 s and 0.8% diethyl sulfate （DES） in 37℃ mutation for 40 min, after screening, we successfully obtained high efficient strain of nitrite degradation, named UV6-DS2, relative to the starting strain, under the condition of 400 mg/L nitrite, after 12 h degradation, nitrite degradation rate in- creased from 92.8% to 97.8% ,to explore its application in food was able to effectively reduce concentration of nitrite in food.

关 键 词：短乳杆菌 紫外诱变 硫酸二乙酯 亚硝酸盐 

分 类 号：TQ920.61[轻工技术与工程—发酵工程]