不同体积分数乙醇沉淀桑黄胞内多糖的理化性质及抗氧化活性  

Physicochemical Properties and Antioxidant Activity of Intracellular Polysaccharides from Phellinus igniarius Precipitated by Different Ethanol Concentrations

作　　者：周慧吉 马海乐[1] 郭丹钊[1] 王振斌[1] 

机构地区：[1]江苏大学江苏省农产品物理加工重点实验室,江苏镇江212013

出　　处：《食品科学》2015年第19期34-38,共5页Food Science

基　　金："十二五"国家科技支撑计划项目(2012BAD36B05);国家自然科学基金青年科学基金项目(31301544);镇江市科技支撑计划工业项目(GY20130018)

摘　　要：研究桑黄胞内多糖醇沉过程中乙醇体积分数对多糖得率、纯度、理化性质和抗氧化活性等的影响。结果表明:随着乙醇体积分数的增加,沉淀粗多糖的得率增加,而粗多糖中多糖含量呈现不规则的变化;不同体积分数乙醇沉淀获得的桑黄多糖中均含有木糖、甘露糖、半乳糖和葡萄糖。扫描电镜图像显示,低体积分数(65%、75%、85%)乙醇沉淀的多糖呈现均匀球状,高体积分数(95%)乙醇沉淀的多糖连成大块片状。桑黄胞内多糖体外抗氧化能力存在明显的量-效关系,95%乙醇沉淀的多糖对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基、2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)自由基的清除效果最好,对Fe^(3+)的还原能力最强,抗氧化活性最高。In this study,the effect of ethanol concentration on the yield,purity,physicochemical properties and antioxidant activity of intracellular polysaccharides（IPs） isolated from Phellinus igniarius was analyzed.Results indicated that with an increase in ethanol concentration,the yield of crude polysaccharides increased,but polysaccharide content of the precipitated crude polysaccharides changed irregularly.Each of the IPs precipitated by different concentrations of ethanol contained xylose,mannose,galactose and glucose.Scanning electron microscopy（SEM） images showed that the IPs precipitated by low concentration（65%、75%、85%） of ethanol presented a uniform spherical shape,whereas those precipitated by high concentration（95%） of ethanol were clustered into large flakes.IPs of Phellinus igniarius had obvious antioxidant activity in vitro in a dose-dependent manner and the IPs precipitated by 95%ethanol had the best scavenging activity against DPPH,hydroxyl and ABTS~＋ free radicals and the strongest ferric reducing antioxidant power.

关 键 词：桑唬黄 胞内多糖 理化性质 体外抗氧化活性 

分 类 号：TS205.9[轻工技术与工程—食品科学]