川皮苷体外抗氧化活性及对肿瘤细胞生长抑制作用  

Antioxidant Activity in vitro of Nobiletin and Its Inhibitory Effect on the Proliferation of Tumor Cells

作　　者：张玉[1,2] 李洪军[1] 窦华亭[3] 贺稚非[1] 

机构地区：[1]西南大学食品科学学院,重庆400715 [2]重庆市特色食品工程技术研究中心,重庆400715 [3]西南大学柑桔研究所,重庆400712

出　　处：《食品科学》2015年第19期233-237,共5页Food Science

基　　金：中央高校基本科研业务费专项资金项目(XDJK2014C071);西南大学教育教学改革研究项目(2013JY062);重庆市北碚区集成示范计划项目(2014-68);重庆市特色食品工程技术研究中心能力提升项目(cstc2014pt-gc8001)

摘　　要：目的:研究川皮苷的抗氧化活性及对肿瘤细胞生长抑制作用。方法:在体外测定川皮苷对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基(·OH)和超氧阴离子自由基(O_2^-·)的清除能力;通过四甲基偶氮唑蓝(methyl thiazolyl tetrazolium,MTT)法筛选对川皮苷作用敏感的肿瘤细胞株,并用倒置显微镜和透射电镜观察敏感细胞株在川皮苷作用之后细胞结构的变化。结果:川皮苷在体外对DPPH自由基、·OH和O_2^-·都具有清除能力,其中对·OH的清除能力突出。同时,人胰腺癌细胞株PANC-1对川皮苷作用敏感,即川皮苷可抑制PANC-1细胞的生长;透射电镜观察发现川皮苷可使PANC-1细胞的细胞核膜破裂,胞质溶出,形成凋亡小体。结论:川皮苷具有显著的体外抗氧化活性,并且可以抑制PANC-1细胞的增殖,在功能食品等领域具有良好的应用前景。Objective：To investigate the antioxidant activity of nobiletin and its inhibitory capacity on the proliferation of cancer cells in vitro.Methods：The scavenging activities of nobiletin on DPPH,hydroxyl and superoxide anion radicals were detected.MTT assay was utilized to evaluate the effect of nobiletin on the growth of different tumor cells.The changes in organelles and cell morphology were observed through inverted microscope and transmission electron microscope.Results：Nobiletin had a strong ability to scavenge DPPH,hydroxyl and superoxide anion radicals,especially to remove hydroxyl radical.Nobiletin had a significant inhibitory effect on the growth of PANC-1 cells.The viability and proliferation of PANC-1 cells were decreased in culture medium with nobiletin.As observed under inverted microscope and transmission electron microscope,nobiletin could result in disruption of the nuclear membrane,release of cytoplasmic contents and generation of apoptotic bodies.Conclusion：Nobiletin has excellent capability to scavenge free radicals and inhibit the proliferation of human PANC-1 cells.

关 键 词：川皮苷 抗氧化 细胞增殖 

分 类 号：R151[医药卫生—营养与食品卫生学]