APC、p53、K-ras在结直肠肿瘤检测中的研究价值  

The Value of APC,p53,K-ras DNA Detection in Colorectal Cancer Screening Study

作　　者：肖丹[1] 钟选芳[1] 许岸高[1] 

机构地区：[1]惠州市第一人民医院消化内科,广东惠州516001

出　　处：《医学综述》2015年第21期3969-3971,共3页Medical Recapitulate

基　　金：广东省医学科研究课题(B2011340)

摘　　要：目的采用聚合酶链反应-单链构象多态性(PCR-SSCP)银染法检测腺瘤多发性息肉病(APC)、p53、K-ras三个基因在结直肠肿瘤筛查中的敏感性和特异性。方法选择2011年11月至2012年8月惠州市第一人民医院消化门诊收治的14例结直肠癌(CRC组),60例结直肠腺瘤(CRA组),30例正常组粪便标本,提取DNA,PCR-SSCP银染法检测基因突变。结果 APC、p53、K-ras三个基因联合检测在CRC组、CRA组、正常组突变率分别为57.1%(8/14)、30.0%(18/60)、3.3%(1/30);在结直肠肿瘤的灵敏度和特异度分别为35.1%(26/74)、96.7%(29/30),粪便DNA联合检测CRC组、CRA组的检出率高于正常组(均P<0.05)。结论粪便DNA联合检测在无创性筛查结直肠肿瘤中具有可行性,可能是一种适合于结直肠肿瘤筛查的无创性方法和筛查模式。Objective To detect the sensitivity and specificity of gene adenomatous polyposis （APC） , p.53, K-ras genetic mutations in colorectal neoplasm using the ploymerase chain reaction-single-strand confer- mational polymorphism（PCR-SSCP） silver staining. Methods Samples were enrolled from individuals who came to Huizhou First People＇s Hospital Gastroenterology Clinic from Nov. 2011 to Aug. 2012：14 cases of colorectal cancer（ CRC group） ,60 cases of coloroctal adenomas（ CRA group） ,30 cases of normal stool speci- mens, DNA was extrated, PCR-SSCP silver staining was used to detect genetic mutations. Results The mutation rates of three genes APC, p.53, K-ras detection in CRC, CRA and normal group were 57.1% （8/ 14） ,30. 0% （18/60） and 3.3% （1/30） respectively. The sensitivity and specificity of three genes detection coloroctal neoplasm were 35.1% （26/74）and 96. 7% （29/30）. The detection rate of three genes in CRC and CRA group were significantly higher than normal group（ all P 〈 0. 05 ）. Conclusion Stool DNA detec- tion is a feasibi]e non-invasive method for screening colorectal neoplasm, which might he a suitable non-inva- sive method for screening colorectal neoplasia.

关 键 词：结直肠肿瘤 粪便DNA 聚合酶链反应-单链构象多态性 腺瘤多发性息肉病基因 p53 K-RAS 

分 类 号：R574.6[医药卫生—消化系统]