木聚糖酶的发酵动力学研究与分批补料控制  

Study on the dynamics of Xylanase fermentation and control of fed-batch

作　　者：肖玲玲[1] 黄瑞[2] 郭金玲[3] 龚大春[3] 

机构地区：[1]湖北三峡职业技术学院,湖北宜昌443002 [2]安琪酵母股份有限公司,湖北宜昌443002 [3]三峡大学艾伦麦克德尔米德再生能源研究所,湖北宜昌443002

出　　处：《可再生能源》2015年第11期1700-1705,共6页Renewable Energy Resources

基　　金：安琪公司横向合作项目(SDHZ2008099)

摘　　要：在里氏木霉发酵产木聚糖酶工艺研究基础上,构建了关于菌体生长、产物形成和底物消耗的发酵动力学方程,并依据发酵动力学过程特点设计了分批补料方案,研究了分批补料发酵过程中生物量、p H值、氨基氮、酶量等参数的变化特征。研究结果表明,分批补料发酵可提高发酵过程的生物量,避免菌体过早衰亡,在120 h后分批补料发酵可以延长菌体产酶时间,提高单位发酵体积的发酵酶活,为工业自动化控制生产奠定良好基础。The fermentation dynamics equations of biomass growth,production formation and substrate consumption were constructed on the basis of the process research on the xylanase production from Trichoderma reesei. According to the dynamics characteristic of the fermentation, the fed-batch fermentation program was designed. The fed-batch performance parameters about biomass,pH value, amino-nitrogen,enzymatic volume were studied. The results showed that the fed-batch process can promote the biomass to avoid the bacterium dying earlier and increase the enzymatic activity with 70%.This research gave a good foundation for the industrial auto control.

关 键 词：里氏木霉 木聚糖酶 发酵动力学 分批补料 

分 类 号：TK6[动力工程及工程热物理—生物能] TQ925[轻工技术与工程—发酵工程]