葡萄酒中10种多酚类物质的高效液相色谱-四极杆/静电场轨道高分辨质谱测定方法研究  

Studies on Determination of 10 Polyphenols in Wine by HPLC- Quadrupole /Electrostatic Field Orbitrap HRMS

作　　者：宓捷波[1] 毕玉国[1] 赵孔祥[1] 李淑静[1] 许泓[1] 何佳[1] 郑文杰[1] 

机构地区：[1]天津出入境检验检疫局,天津300461

出　　处：《分析测试学报》2015年第11期1227-1232,共6页Journal of Instrumental Analysis

基　　金：国家质检总局科技资助项目(2014IK126)

摘　　要：利用高分辨质谱的定性鉴别能力,建立了葡萄酒中10种多酚类物质含量的高效液相色谱-四极杆/静电场轨道高分辨质谱（HPLC-QE HRMS）分析方法。样品经酸化后乙酸乙酯提取,ACQUITY UPLC BEH C18色谱柱（2.1 mm×100 mm,1.7μm）分离,全扫描和目标离子二级扫描定性,外标法定量。10种多酚类物质的定量下限为0.000 5~0.002 5 mg/L,加标回收率为82.0%~109.1%,相对标准偏差（RSD）小于10%。A method of HPLC- quadrupole / electrostatic field orbitrap HRMS（ HPLC- QE HRMS）was established to analyze 10 polyphenols in wine with the help of qualitative confirmation of HRMS.The samples were extracted with ethyl acetate after acidized. The qualitative confirmation was carried out by full scan and target MS2 spectra after separation on an ACQUITY UPLC BEH C18column（ 2. 1mm × 100 mm,1. 7 μm）. An external standard method was used for quantitative analysis. The limits of quantitation（ LOQs） of 10 polyphenols ranged from 0. 000 5 mg / L to 0. 002 5 mg / L. The spiked recoveries were between 82. 0% and 109. 1% with RSDs less than 10%.

关 键 词：高效液相色谱(HPLC) 高分辨质谱 多酚 葡萄酒 

分 类 号：O657.63[理学—分析化学] O625.31[理学—化学]