鞘氨醇单胞菌TP-3原生质体制备与再生的研究  

Conditions for protoplast preparation and regeneration by Sphingomonas sanxanigenens TP-3

作　　者：周明明[1] 李晓雁[2] 陈悦[2] 陈彦梅[1] 黄海东[1] 

机构地区：[1]天津农学院农学与资源环境学院,天津300384 [2]天津农学院食品科学与生物工程学院,天津300384

出　　处：《食品工业科技》2015年第22期184-188,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(31571790);天津市大学生创新创业项目(201410061062)

摘　　要：研究了EDTA预处理、菌龄、溶菌酶浓度、酶解温度及酶解时间对鞘氨醇单胞菌TP-3原生质体制备与再生的影响。结果表明:10 mmol/L EDTA对菌体进行预处理,菌龄18 h,酶浓度60μg/m L,酶解温度32℃,酶解时间30 min时,菌株原生质体的形成率可达96.1%,再生率达32.2%。本文为进行原生质体融合选育优良微生物菌种奠定了基础。The effect of EDTA pretreatment,cell age and the concentration,temperature and work time of lysozyme on protoplast formation and regeneration by Sphingomonas sanxanigenens TP-3 were studied. The results showed that when cell age was 18 h,pretreated by 10 mmol/L EDTA,using 60 μg/m L of lysozyme,enzymolysis at 32 ℃ for 30 min,the protoplast formation rate and regeneration rate respectively reached 96.1% and 32.2%.This work lay the foundation for protoplast fusion to breed good microbial strain.

关 键 词：鞘氨醇单胞菌 原生质体 影响因素 

分 类 号：TS201.1[轻工技术与工程—食品科学]