有机溶剂分级沉淀分离线性β-1,3-葡寡糖  

Separation of linear β-1,3-glucooligosaccharides by fractional precipitation of organic solvents

作　　者：陆光兴 罗宸 毛江川 杨晗[1] 贾云[1] 蒋巧诗 张洪涛[1] 詹晓北[1,2] 朱莉 

机构地区：[1]江南大学生物工程学院,糖化学与生物技术教育部重点实验室,江苏无锡214122 [2]江苏瑞光生物科技有限公司,江苏无锡214125

出　　处：《食品工业科技》2015年第22期256-261,共6页Science and Technology of Food Industry

基　　金：国家级大学生创新创业训练计划;国家自然科学基金(31171640,31201384,31271888);无锡市科技支撑计划(CLE01N1208);无锡市生物农业领军型创业人才计划(“130”计划);无锡中小企业创新基金(CBE01G1344)

摘　　要：盐酸水解二甲亚砜中的热凝胶可大规模地制备线性β-1,3-葡寡糖。为了快速高效地分离二甲亚砜中线性β-1,3-葡寡糖,采用不同种类的有机溶剂进行沉淀和分级沉淀。十三种常见有机溶剂中九种可以沉淀热凝胶水解产物,其中二氯甲烷和乙酸乙酯具有较高的应用可行性。二氯甲烷和乙酸乙酯可以分级沉淀分离线性β-1,3-葡寡糖,乙酸乙酯使用体积较小但选择性较差。分级沉淀效果受热凝胶浓度、水含量和离子强度的影响,而不受水解程度的影响。有机溶剂分级沉淀法可以快速高效地终止水解反应和分离水解产物,并可进一步回收二甲亚砜和有机溶剂进行重复利用,对β-1,3-葡寡糖的分离纯化具有重要的指导意义。HCl hydrolysis of Curdlan in dimethyl sulfoxide could massively prepare linear β- 1, 3-glucooligosaccharides. Different kinds of organic solvents were selected to quickly and efficiently separate linear β-1,3-glucooligosaccharides from dimethyl sulfoxide by precipitation and fractional precipitation. Nine kinds of common organic solvents could precipitate Curdlan hydrolysis product,and high feasibility could be got by methylene chloride and ethyl acetate. Methylene chloride and ethyl acetate could be used to separate linear β-1,3-glucooligosaccharides by fractional precipitation and smaller volume but poor selectivity with ethyl acetate. Curdlan concentration,water content and ionic intensity had different influences on fractional precipitation,but degree of hydrolysis had no influence on fractional precipitation. Organic solvent fractional precipitation method could terminate Curdlan hydrolysis reaction and separate hydrolysis product efficiently and quickly. Moreover, it was conducive to recycle dimethyl sulfoxide and organic solvent. Therefore, this method had important guiding significance for separation and purification of β-1,3-glucooligosaccharides.

关 键 词：热凝胶 线性β-1 3-葡寡糖 二甲亚砜 有机溶剂 分级沉淀 

分 类 号：TS245.9[轻工技术与工程—制糖工程]