不同形态硒和复配对大鼠不同组织硒沉积规律的影响  

Selenium deposition regularity with different forms of selenium in rat tissues

作　　者：秦燕[1] 邓泽元[1] 姚昭[1] 李红艳[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《南昌大学学报（理科版）》2015年第3期275-279,295,共5页Journal of Nanchang University(Natural Science)

基　　金：江西省科技厅国际合作项目(20122BDH80003);江西省博士后科研项目(2013KY04)

摘　　要：探究大鼠摄食不同形态硒和复配后,肾脏、肝脏、心脏和骨骼中硒的沉积规律。大鼠连续灌胃有机硒(LSeMC),无机硒(亚硒酸钠),有机硒+VE,L-SeMC+VE+原花青素等30d后,采集其肾脏、肝脏、心脏和骨骼,经原子荧光光度法测定各组织总硒含量。结果显示:添加不同形态的硒对大鼠各组织硒沉积量均有所提高;有机硒与VE联用组硒含量比单独添加有机硒或无机硒组有显著性提高(P<0.05),表明VE可促进硒在组织中的沉积;L-SeMC+VE+原花青素组大鼠组织中的硒沉积量与硒的添加量成正相关关系,但其大鼠组织中硒沉积量低于其他同等剂量硒添加组,表明原花青素可能会降低硒在组织中的沉积量。结论:添加不同形式的硒均能提高大鼠组织硒的沉积量;有机硒与VE联用能明显促进组织中硒元素的沉积;在一定范围内,硒在组织中的沉积量随含硒剂量的增加而升高。The current stuy aimed to investigate the deposition regularity of selenium on rat＇s kidney,liver,heart and bones after been fed with different forms of selenium.The rats were fed by gavage once a day for4 weeks with organic selenium（L-SeMC）,inorganic selenium（Na2SeO3）,vitamin E and supplements（containing L-SeMC、Na2SeO3 and procyanidins）,respectively.The samples of kidney,liver,heart and bones from the sacrificed rats were collected and digested by a wet digestion protocol.Total selenium content was determined by Atomic Fluorescence Spectrometry（AFS）.In this study,we found that the deposition of selenium in rats tissues increased after fed with the two forms of selenium in the experiment.Moreover,when compared with organic selenium/VE group,organic group and VE group,the total selenium content in the selenium/VE group was significantly higher than the other two groups except in kidney（P〈0.05）,indicating that VE accelerated the deposition of selenium in rat tissues.Different doses of supplement groups revealed that the tissues selenium content was proportional to the dose of supplements.But the selenium deposition in supplements group was lower than the organic/inorganic group with the same dose of selenium.It could be speculated that procyanidins might inhibit the deposition of selenium in tissues.In conclusion,different forms of selenium exhibited good effects of selenium deposition in rat tissues.Combination of organic selenium and VE significantly increased the total selenium content.Within certain limits,the tissue selenium content increased with the dose of selenium raised.

关 键 词：有机硒 无机硒 VE 复配 原子荧光光度法 

分 类 号：Q95-33[生物学—动物学]