米醋废渣发酵红曲色素的条件研究  

Fermentation conditions of Monascus pigments with vinegar wastes

作　　者：杜甫军 胡伟莲 冯纬 戴德慧[2] 

机构地区：[1]杭州食品酿造有限公司,浙江杭州310017 [2]浙江科技学院,浙江杭州310023

出　　处：《中国酿造》2015年第11期71-74,共4页China Brewing

基　　金：浙江省科技计划项目(2014C33039)

摘　　要：对米醋废渣主要成分进行了分析,其中干燥质量损失为66.89%,酒精度为4.15%vol,干物质中粗蛋白、粗淀粉、粗纤维、粗脂肪含量分别为63.83%、7.55%、10.77%、9.02%。以米醋废渣为发酵培养基对8株红曲霉进行了筛选,得到一株红曲色素产量较高的红曲霉MS-5,其色素合成周期显著短于同条件下的大米培养基。通过单因素及正交试验对米醋废渣红曲色素发酵工艺进行了优化,结果表明,最佳工艺参数为葡萄糖添加量5%,氯化铵添加量1.5%,初始水含量50%,初始p H值6.0,发酵时间6 d。在此条件下红曲色素发酵产量可达(1 252.5±47)U/g。The main components of vinegar wastes were analyzed. The results showed that loss on drying, alcohol content, crude protein, crude starch, crude fiber, crude fat were 66.89%, 4.15%vol, 63.83%, 7.55%, 10.77% and 9.02%, respectively. Using vinegar wastes as fermentation medium, a strain named Monascus sp. MS-5 with high-yield Monascus pigments was screened from eight strains, and the pigments synthesizing cycle reduced significantly compared with the rice medium in the same condition. The optimal fermentation conditions of vinegar waste Monascus pigments were op- timized by single factor and orthogonal experiments. Results indicated that the optimal technology parameters were glucose 5%, ammonium chloride 1.5%, original moisture content 50%, original pH 6.0 and fermentation time 6 d. The yield of Monascus pigments reached up to （1 252.5±47） U/g at the optimal condition.

关 键 词：米醋废渣 红曲色素 固态发酵 优化 

分 类 号：Q933-31[生物学—微生物学]