柱层析法连续提取葡萄籽油和多酚的工艺技术  

Sequential Extraction of Oil and Polyphenols from Grape Seed by Column Chromatography Method

作　　者：王明阳 李璠 解瑞林 韩寒冰[2] 刘杰凤[2] 

机构地区：[1]茂名出入境检验检疫局,广东茂名525000 [2]广东石油化工学院环境与生物工程学院,广东茂名525000

出　　处：《食品研究与开发》2015年第19期122-125,共4页Food Research and Development

基　　金：广东省科技计划项目(2013B020503069)

摘　　要：研究连续提取并分离葡萄籽中的葡萄籽油和多酚的最佳工艺条件。将干燥的葡萄籽粉末用石油醚湿润装进层析柱中,浸泡2 h,先后用石油醚和60%乙醇溶液连续洗脱,连续收集石油醚6倍体积(m L/g)和60%乙醇溶液10倍体积洗脱液,混合洗脱液分层,醚层中含有葡萄籽油,下层醇水液中含有多酚。连续提取的葡萄籽油和多酚的提取率均达到99%,经萃取一次性分离葡萄籽油和多酚,得率分别是97.6%和98.0%。利用柱层析连续提取葡萄籽油和多酚,并通过萃取可把两种成分一次性分离开来,工艺简单,时间短,有机溶剂用量少,提取效率高,具有广泛应用价值。To explore technical process and conditions for the sequential extraction of grape seed oil and polyphenol by using column chromatography from grape seed. Dried material was loaded into a column with petroleum ether and sequentially eluted with 6-fold（m L/g） petroleum ether, 10-fold 60 %（v/v） ethanol. The elutes was separated into an ether fraction containing grape seed oil and an ethanol-water fraction containing polyphenol. Through this procedure, grape seed oil and polyphenol in grape seed were simultaneously extracted at 99 % extraction rates and simply separated at higher than 97.6 % and 98.0 % recovery rates. The process was simple and short, with less consumption of solvents and high extraction rates. The method provides a simple and high-efficient extraction and separation of wide range bioactive substances.

关 键 词：葡萄籽 柱层析 连续提取 葡萄籽油 多酚 

分 类 号：TS261.9[轻工技术与工程—发酵工程]