海洋细菌Pseudoalteromonas issachenkonii产胞外多糖EPSPⅡ的发酵工艺优化  

Optimization of fermentation conditions for exopolysaccharide EPS- PⅡby marine bacterium Pseudoalteromonas issachenkonii

作　　者：郝鲁江[1] 范秋苹 张晓飞[1] 卢晓平[1] 许艳蕊[1] 

机构地区：[1]齐鲁工业大学山东省微生物工程重点实验室,山东济南250353

出　　处：《食品工业科技》2015年第23期256-260,共5页Science and Technology of Food Industry

基　　金：山东省自然科学基金(ZR2012CM019)

摘　　要：目的:本实验对一株产胞外多糖EPS-PⅡ的海洋细菌Pseudoalteromonas issachenkonii的发酵条件进行优化。方法:采用单因素实验,确定培养基组成,并采用Plackett-Burman联用Box-Behnken设计确定最佳发酵条件。结果:实验确定发酵培养基由葡萄糖,酵母浸膏和海盐组成,发酵条件为培养时间33.8 h,接种量8.8%,摇床转速170 r/min。结论:采用最优发酵条件得出EPS-PⅡ最高产量可达2.62 g/L,比优化前提高了116.5%。Objective： To optimize the fermentation conditions of exopolysaccharide EPS- P Ⅱ produced by Pseudoalteromonas issachenkonii.Method：The medium composition was determined by single factor experiment, Plackett- Burman combined with Box- Behnken was used to determine the optimum fermentation conditions.Results：The experiment showed that the fermentation medium was composed of glucose, yeast extract and baysalt, the fermentation conditions were 8.8 % inoculum, 170 r/min shaking speed and 33.8 h for fermentation. Conclusion：Under the optimum conditions,the yield of EPS-P Ⅱ was 2.62 g/L,which was increased by 116.5%.

关 键 词：海洋细菌 胞外多糖 单因素实验 Plackett-Burman法 Box-Behnken法 

分 类 号：TS201.1[轻工技术与工程—食品科学]