牡丹花蕊水提取液抗氧化功能研究  

Antioxidant activities of aqueous extract from peony(Paeonia suffruticosa Andr.) flower

作　　者：李朝苹[1] 陈立勇[2] 阴英超 徐贵法[1] 

机构地区：[1]山东大学公共卫生学院营养与食品卫生研究所,山东济南250012 [2]山东大学附属省立医院营养科,山东济南250021 [3]菏泽尧舜牡丹生物科技有限公司,山东菏泽274000

出　　处：《山东大学学报（医学版）》2015年第11期32-36,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81470498)

摘　　要：目的探讨牡丹花蕊水提取液的抗氧化功能。方法采用Al Cl3比色法测定牡丹花蕊水提取液的总黄酮含量,采用福林酚法测定总多酚含量;通过ABTS和DPPH自由基清除法评价牡丹花蕊水提取液的体外抗氧化能力。通过一次性灌胃50%乙醇(12 m L/kg)诱导过氧化小鼠模型,并测定血清和肝脏超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量,评价牡丹花蕊水提取液的体内抗氧化作用。结果牡丹花蕊水提取液总黄酮含量为(15.81±2.66)mg芦丁/g;总酚酸含量为(46.63±5.05)mg没食子酸/g。牡丹花蕊水提取液具有较强的清除ABTS和DPPH自由基的作用。动物实验表明灌胃给予500 mg/(kg·d)牡丹花蕊水提取液30 d能显著提高乙醇诱导的过氧化损伤小鼠血清和肝脏抗氧化酶活力(P<0.05),显著抑制MDA含量的升高(P<0.05)。结论牡丹花蕊水提取液含有生物活性物质黄酮和总酚酸,其在体内外抗氧化功效中发挥重要作用。Objective To explore the anti-oxidative effect of aqueous extract from peony flower. Methods The total flavonoid content in aqueous extract was determined by the AlCl3 colorimetric method, and the total phenolic content was determined by the Folin-Ciocalteu method. The in vitro antioxidant abilities of peony flower aqueous extract were esti- mated by ABTS and DPPH radical scavenging activity. Its in vivo antioxidant ability was evaluated according to the su- peroxide dismutase （SOD） and glutathione peroxidase （GSH-Px） activities, and malondialdehyde （MDA） content in serum and liver tissue of mice model with peroxidation induced by gastric gavage of 50% ethanol （12 mL/kg） for one time. Results The flavonoid content of aqueous extract from peony flower is （ 15.81 ±2.66 ） mg rutin/g and total phenolic acid content is （ 46.63 ± 5.05 ） mg gallic acid/g. Extracts showed potent antioxidant activity according to ABTS, DPPH free radical scavenging method. Oral administration with high dose of aqueous extract of peony flower [500 mg/（kg· d）] for 30 days significantly improved activity of antioxidant enzymes （P 〈 0.05 ） and reduced the content of MDA （ P 〈 0.05 ） in serum and liver tissue of the ethanol-induced peroxidation mice model. Conclusion The peony flower extracts with high total phenolic and flavonoid content possess remarkable antioxidant capacity.

关 键 词：牡丹花蕊 抗氧化能力 自由基清除 抗氧化酶 丙二醛 

分 类 号：R151.3[医药卫生—营养与食品卫生学]