金属离子对尖孢镰刀菌发酵液中果胶酶活性的影响  

Effect of Metal Ions on Pectic Enzyme Activity in Fusarium Oxysporum Fermentation

作　　者：李文强[1] 吴菲菲[1,2,3] 李化强[1,2,3] 赵良忠[1] 卜宇芳 徐纬[1] 彭玲慧 尹锦辉 

机构地区：[1]邵阳学院生物与化学工程系,湖南邵阳422000 [2]大连理工大学生命科学与生物技术学院教育部动物性食品安全保障技术工程研究中心,辽宁大连116024 [3]大连赛姆生物工程技术有限公司博士后工作站,辽宁大连116620

出　　处：《邵阳学院学报（自然科学版）》2015年第4期50-56,共7页Journal of Shaoyang University：Natural Science Edition

基　　金：湖南省科技厅项目(2014FJ4253);湖南省教育厅项目(14C1022)

摘　　要：采用发酵产酶法脱脐橙囊衣过程中,为提高酶活在培养基中添加不同金属离子,接种尖孢镰刀菌经摇瓶发酵后,测定发酵液中果胶酶系三个组分酶(聚半乳糖醛酸酶PG、果胶酸裂解酶PL、果胶酯酶PE)酶活性,探讨不同金属离子种类、添加量对果胶酶活性的影响.实验结果表明:浓度为0.06%Mg^(2+)、0.04%Fe^(2+)提高PG酶活力达205.62 U/m L、297.33 U/m L,分别比对照组提高1.07倍、1.45倍;Fe^(2+)浓度为0.08%时,PL酶活力达到283.31 U/m L,是对照组的2.83倍,Fe^(2+)对PL酶活力起促进作用;Fe^(2+)、Zn^(2+)对PE酶活力的影响有显著的促进作用,浓度为0.06%时达到最大值255.67 U/m L、200.68 U/m L,是对照组的2.40倍和1.66倍.本实验结果对优化橙汁胞生产工艺,实现安全高效低成本去除柑橘囊衣提供参考.Through adding different metal ions in the culture medium,inoculated fusarium oxysporum after shaking flask fermentation,determination of pectinase are three components in fermented liquid enzyme( Polygalactuonatelyase PG,Polygalactuonatelyase PL,Petinesterase PE) enzyme activity,discuss different types of metal ions,adding amount on the influence of the pectic enzyme activity. The experimental results show that 0. 06% Mg2+,0. 04% Fe2+raise PG enzyme activity of 205. 62 U / m L,297. 33 U / m L,1. 07 times,1. 45 times higher than that of control group,respectively. When Fe2+concentration was 0. 08%,PL enzyme activity reached 283. 31 U /m L,2. 83 times of the control group,indicating Fe2+plays a role of promoting PL enzyme activity. Fe2+,Zn2+have a significant role in promoting PE enzyme activity,the influence of the concentration of 0. 06% when the maximum 255. 67 U / m L,200. 68 U / m L,2. 40 times and 1. 66 times of the control group. The experimental results of metal ions enhance the vitality of pectinase different components has the reference value,in order to improve the enzyme activity to make it more efficient,fast,the degradation of citrus sacs,realize industrialization of fruit and vegetable cleaning and processing and production to provide the reference.

关 键 词：果胶酶 金属离子 尖孢镰刀菌 聚半乳糖醛酸酶(PG) 果胶裂解酶(PL) 果胶酯酶(PE) 

分 类 号：TS201.3[轻工技术与工程—食品科学]